DNA, cell wall and general oxidative damage underlie the tellurite/cefotaxime synergistic effect in Escherichia coli
dc.contributor.author | Molina-Quiroz, R. | |
dc.contributor.author | Loyola, D. | |
dc.contributor.author | Muñoz-Villagrán, C. | |
dc.contributor.author | Quatrini, R. | |
dc.contributor.author | Vásquez, C. | |
dc.contributor.author | Pérez-Donoso, J. | |
dc.date.accessioned | 2023-04-04T15:49:39Z | |
dc.date.available | 2023-04-04T15:49:39Z | |
dc.date.issued | 2013-11 | |
dc.description | Indexación: Scopus. | |
dc.description.abstract | The constant emergence of antibiotic multi-resistant pathogens is a concern worldwide. An alternative for bacterial treatment using nM concentrations of tellurite was recently proposed to boost antibiotic-toxicity and a synergistic effect of tellurite/cefotaxime (CTX) was described. In this work, the molecular mechanism underlying this phenomenon is proposed. Global changes of the transcriptional profile of Escherichia coli exposed to tellurite/CTX were determined by DNA microarrays. Induction of a number of stress regulators (as SoxS), genes related to oxidative damage and membrane transporters was observed. Accordingly, increased tellurite adsorption/uptake and oxidative injuries to proteins and DNA were determined in cells exposed to the mixture of toxicants, suggesting that the tellurite-mediated CTX-potentiating effect is dependent, at least in part, on oxidative stress. Thus, the synergistic tellurite-mediated CTX-potentiating effect depends on increased tellurite uptake/adsorption which results in damage to proteins, DNA and probably other macromolecules. Our findings represent a contribution to the current knowledge of bacterial physiology under antibiotic stress and can be of great interest in the development of new antibiotic-potentiating strategies. | es |
dc.description.uri | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0079499 | |
dc.identifier.citation | PLoS ONE, Volume 8, Issue 1118, November 2013, Article number e79499 | es |
dc.identifier.doi | 10.1371/journal.pone.0079499 | |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://repositorio.unab.cl/xmlui/handle/ria/48174 | |
dc.language.iso | en | es |
dc.publisher | Public Library of Science | es |
dc.rights.license | Attribution 4.0 International (CC BY 4.0) | |
dc.rights.uri | https://journals.plos.org/plosone/s/journal-information#loc-open-access | |
dc.subject | Anti-Bacterial Agents | es |
dc.subject | Cefotaxime | es |
dc.subject | Cell Wall | es |
dc.subject | Escherichia coli | es |
dc.subject | Tellurium | es |
dc.title | DNA, cell wall and general oxidative damage underlie the tellurite/cefotaxime synergistic effect in Escherichia coli | es |
dc.type | Artículo | es |
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