Degradación de cloroformo por la bacteria antártica : pseudomonas veronii 68
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Archivos
Fecha
2024
Profesor/a Guía
Facultad/escuela
Idioma
es
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Universidad Andrés Bello
Nombre de Curso
Licencia CC
Licencia CC
Resumen
El cloroformo es un compuesto tóxico y mutagénico. Es empleado en la industria para inhibir el crecimiento de microorganismos, causando intoxicación por especies reactivas de oxígeno y lisis celular.
A la fecha, son escasos los reportes de bacterias capaces de tolerar y degradar el cloroformo. Se ha descrito la degradación de este compuesto mediante procesos de co-metabolismo del tolueno, butano, amoniaco y metano, mediado principalmente por enzimas monooxigenasas. Todos estos estudios utilizan bajas concentraciones de cloroformo, las cuales van de 0,00002 a 0,0007% p/v. Por otro lado, a la fecha no se ha descrito bacterias capaces de utilizar este compuesto como única fuente de carbono.
En este trabajo, estudiamos la capacidad de una bacteria antártica, Pseudomonas veronii 68, de resistir altas concentraciones de cloroformo y utilizarlo como única fuente de carbono. Para esto se midió el crecimiento bacteriano a través de Unidades Formadoras de Colonias (UFC), se cuantificó las proteínas intracelulares y se determinó la actividad biológica de los cultivos. También se realizó una medición de los cloruros liberados en el medio, que se generan como subproducto de la degradación del cloroformo.
Los resultados sugieren que esta bacteria es capaz de crecer utilizando cloroformo como única fuente de carbono. A su vez, se observó un mayor crecimiento en los cultivos suplementados con 10% y 1% p/v, lo cual se correlacionó con un aumento en la cantidad de proteínas y actividad biológica. Adicionalmente, se determinó un aumento en la concentración de cloruro liberado al medio, que podría deberse a una degradación del cloroformo.
En conclusión, los resultados obtenidos sugieren que P. veronii 68 es capaz de tolerar altas concentraciones de cloroformo y utilizarlo como única fuente de carbono, siendo el primer microorganismo con esta capacidad descrito a la fecha, y que podría ayudar a biorremediar aguas o suelos contaminados por este compuesto.
Chloroform is a toxic and mutagenic compound. It is utilized in the industry to hinder the proliferation of microorganisms, resulting in intoxication by reactive oxygen species and cellular lysis. To date, there have been scarce reports of bacteria capable of tolerating and degrading chloroform. The degradation of this compound has been elucidated through co-metabolism processes involving toluene, butane, ammonia, and methane, primarily mediated by monooxygenase enzymes. All these studies employ low concentrations of chloroform, ranging from 0.00002 to 0.0007% w/v. Conversely, as of now, bacteria proficient in utilizing this compound as the exclusive carbon source have not been described. In this study, we investigated the proficiency of an Antarctic bacterium, P. veronii 68, in enduring high concentrations of chloroform and utilizing it as the unique carbon source. To this end, bacterial growth was gauged through Colony Forming Units (CFU), intracellular proteins were quantified, and the biological activity of the cultures was ascertained. Furthermore, a quantification of the chlorides liberated in the medium, which arise as a byproduct of chloroform degradation, was undertaken. The findings demonstrated that this bacterium is proficient in thriving using chloroform as carbon source. Furthermore, heightened growth was observed when the cultures were supplemented with 10% and 1% w/v, which exhibited a positive correlation with an escalation in the quantity of proteins and biological activity. Additionally, an augmentation in the concentration of liberated chloride into the medium was established. In conclusion, altogether these results indicate that P. veronii 68 possesses the capability to withstand elevated concentrations of chloroform and employ it as the exclusive carbon source. It stands as the inaugural microorganism with this attribute documented to date, holding potential promise in the remediation of water or soil tainted by this compound.
Chloroform is a toxic and mutagenic compound. It is utilized in the industry to hinder the proliferation of microorganisms, resulting in intoxication by reactive oxygen species and cellular lysis. To date, there have been scarce reports of bacteria capable of tolerating and degrading chloroform. The degradation of this compound has been elucidated through co-metabolism processes involving toluene, butane, ammonia, and methane, primarily mediated by monooxygenase enzymes. All these studies employ low concentrations of chloroform, ranging from 0.00002 to 0.0007% w/v. Conversely, as of now, bacteria proficient in utilizing this compound as the exclusive carbon source have not been described. In this study, we investigated the proficiency of an Antarctic bacterium, P. veronii 68, in enduring high concentrations of chloroform and utilizing it as the unique carbon source. To this end, bacterial growth was gauged through Colony Forming Units (CFU), intracellular proteins were quantified, and the biological activity of the cultures was ascertained. Furthermore, a quantification of the chlorides liberated in the medium, which arise as a byproduct of chloroform degradation, was undertaken. The findings demonstrated that this bacterium is proficient in thriving using chloroform as carbon source. Furthermore, heightened growth was observed when the cultures were supplemented with 10% and 1% w/v, which exhibited a positive correlation with an escalation in the quantity of proteins and biological activity. Additionally, an augmentation in the concentration of liberated chloride into the medium was established. In conclusion, altogether these results indicate that P. veronii 68 possesses the capability to withstand elevated concentrations of chloroform and employ it as the exclusive carbon source. It stands as the inaugural microorganism with this attribute documented to date, holding potential promise in the remediation of water or soil tainted by this compound.
Notas
Tesis (Licenciado en Biología)
Palabras clave
Cloroformo, Pruebas, Pseudomonas, Biorremediación.