Regulation of a novel Acidithiobacillus caldus gene cluster involved in metabolism of reduced inorganic sulfur compounds

dc.contributor.authorRzhepishevska, Olena I.
dc.contributor.authorValdes, Jorge
dc.contributor.authorMarcinkeviciene, Liucija
dc.contributor.authorGallardo, Camelia Algora
dc.contributor.authorMeskys, Rolandas
dc.contributor.authorBonnefoy, Violaine
dc.contributor.authorHolmes, David S.
dc.contributor.authorDopson, Mark
dc.date.accessioned2024-07-29T19:44:01Z
dc.date.available2024-07-29T19:44:01Z
dc.date.issued0027-11-01
dc.descriptionINDEXACIÓN: WEB OF SCIENCE.
dc.description.abstractAcidithiobacillus caldus has been proposed to play a role in the oxidation of reduced inorganic sulfur compounds (RISCs) produced in industrial biomining of sulfidic minerals. Here, we describe the regulation of a new cluster containing the gene encoding tetrathionate hydrolase (tetH), a key enzyme in the RISC metabolism of this bacterium. The cluster contains five cotranscribed genes, ISac1, rsrR, rsrS, tetH, and doxD, coding for a transposase, a two-component response regulator (RsrR and RsrS), tetrathionate hydrolase, and DoxD, respectively. As shown by quantitative PCR, rsrR, tetH, and doxD are upregulated to different degrees in the presence of tetrathionate. Western blot analysis also indicates upregulation of TetH in the presence of tetrathionate, thiosulfate, and pyrite. The tetH cluster is predicted to have two promoters, both of which are functional in Escherichia coli and one of which was mapped by primer extension. A pyrrolo-quinoline quinone binding domain in TetH was predicted by bioinformatic analysis, and the presence of an o-quinone moiety was experimentally verified, suggesting a mechanism for tetrathionate oxidation.
dc.description.urihttps://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2168230&blobtype=pdf
dc.identifier.citationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY, Volume 73, Issue 22, Page 7367-7372
dc.identifier.doi10.1128/AEM.01497-07
dc.identifier.issn0099-2240
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/58820
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.subjectACID-MINE DRAINAGE
dc.subjectTHIOBACILLUS-FERROOXIDANS
dc.subjectFERROBACILLUS-FERROOXIDANS
dc.subjectTETRATHIONATE HYDROLASE
dc.subjectQUINONE OXIDOREDUCTASEOXIDIZING BACTERIAESCHERICHIA-COLISULFIDE MINERALSOXIDATIONTHIOSULFATE
dc.subjectOXIDIZING BACTERIA
dc.subjectESCHERICHIA-COLI
dc.subjectSULFIDE MINERALS
dc.subjectOXIDATION
dc.subjectTHIOSULFATE
dc.titleRegulation of a novel Acidithiobacillus caldus gene cluster involved in metabolism of reduced inorganic sulfur compounds
dc.typeArtículo
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