Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA

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Fecha
2002-07-02
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
OXFORD UNIV PRESS
Nombre de Curso
Licencia CC
CC BY-NC 4.0 ATRIBUCIÓN-NOCOMERCIAL 4.0 INTERNACIONAL Deed
Licencia CC
https://creativecommons.org/licenses/by-nc/4.0/deed.es
Resumen
Saccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xylose, but not glucose. Analysis by pulse-field gel electrophoresis and sequencing indicates that xynA, including its eight introns, has been inserted into the yeast genome. It was shown by sequencing that clone 44A is able to correctly splice xynA introns. This is the first successful attempt to express a fungal endoxylanase gene in yeast with correct intron splicing. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Notas
INDEXACIÓN: WEB OF SCIENCE.
Palabras clave
xylanases, heterologous gene expression, Penicillium purpurogenum, Saccharomyces cerevisiae
Citación
FEMS MICROBIOLOGY LETTERS, Volume212, Issue 2, Page 237-241
DOI
10.1111/j.1574-6968.2002.tb11272.x
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