Secretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA

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dc.contributor.authorChávez, R
dc.contributor.authorNavarro, C
dc.contributor.authorCalderón, I
dc.contributor.authorPeirano, A
dc.contributor.authorBull, P
dc.contributor.authorEyzaguirre, J
dc.date.accessioned2024-07-03T14:25:23Z
dc.date.available2024-07-03T14:25:23Z
dc.date.issued2002-07-02
dc.descriptionINDEXACIÓN: WEB OF SCIENCE.
dc.description.abstractSaccharomyces cerevisiae was transformed with a genomic library from Penicillium purpurogenum, and an endoxylanase-producing yeast clone (named 44A) that grows on xylose or xylan as sole carbon source was isolated. This yeast synthesizes xynA mRNA and secretes endoxylanase A to culture media when grown on xylan or xylose, but not glucose. Analysis by pulse-field gel electrophoresis and sequencing indicates that xynA, including its eight introns, has been inserted into the yeast genome. It was shown by sequencing that clone 44A is able to correctly splice xynA introns. This is the first successful attempt to express a fungal endoxylanase gene in yeast with correct intron splicing. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
dc.identifier.citationFEMS MICROBIOLOGY LETTERS, Volume212, Issue 2, Page 237-241
dc.identifier.doi10.1111/j.1574-6968.2002.tb11272.x
dc.identifier.issn0378-1097
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/58174
dc.language.isoen
dc.publisherOXFORD UNIV PRESS
dc.rights.licenseCC BY-NC 4.0 ATRIBUCIÓN-NOCOMERCIAL 4.0 INTERNACIONAL Deed
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/deed.es
dc.subjectxylanases
dc.subjectheterologous gene expression
dc.subjectPenicillium purpurogenum
dc.subjectSaccharomyces cerevisiae
dc.titleSecretion of endoxylanase A from Penicillium purpurogenum by Saccharomyces cerevisiae transformed with genomic fungal DNA
dc.typeArtículo
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