Detection and quantification of sars-cov-2 wastewater treatment plants from different cities in chile; towards to a permanent sentinel surveillance
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Fecha
2022
Profesor/a Guía
Facultad/escuela
Idioma
es
Título de la revista
ISSN de la revista
Título del volumen
Editor
Sociedad Chilena de Infectologia
Nombre de Curso
Licencia CC
Attribution-NonCommercial 4.0 International
CC BY-NC 4.0
Deed
Licencia CC
https://creativecommons.org/licenses/by-nc/4.0/deed.en
Resumen
Background: The quantification of SARS-CoV-2 in wastewater is a tool that allows determining the trend of viral circulation in a particular geographical area. Aim: To quantify the SARS-CoV-2 virus in 15 wastewater treatment plants in different Chilean cities to establish a comparison with the variables of: I) Active cases per 100,000 inhabitants; ii) daily positivity (novel cases); and iii) phases of the lockdown strategy. Methods: SARS-CoV-2 was concentrated from wastewater samples. To obtain the number of virus genomes per liter, absolute quantification was performed using qRT-PCR. Results: Between January and June 2021, 253 samples were processed, all of which were positive for the presence of the virus. Likewise, it will be determined that the rate of active cases per 100,000 inhabitants is the variable that best fits the trends obtained with the quantification of the viral load in wastewater. Conclusions: The quantification of SARS- CoV-2 in wastewater as a continuous strategy is an efficient tool to determine the trend of the viral circulation in a delimited geographical area and, combined with genomic surveillance, it can constitute an ideal sentinel surveillance alert on future outbreaks. © 2022, Sociedad Chilena de Infectologia. All rights reserved.
Notas
Indexación: Scopus.
Palabras clave
Active case rate, Genomic surveillance, SARS-CoV-2, Sentinel surveillance, Wastewater-based epidemiology WBE
Citación
Revista Chilena de Infectologia, Volume 39, Issue 6, Pages 690 - 698, 2022
DOI
10.4067/S0716-10182022000600690