Detection and quantification of sars-cov-2 wastewater treatment plants from different cities in chile; towards to a permanent sentinel surveillance

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dc.contributor.authorOlivares-Pacheco, Jorge
dc.contributor.authorAdell, Aiko D.
dc.contributor.authorHepp, Matias I.
dc.contributor.authorReis, Andressa S.
dc.contributor.authorEcheverria, César
dc.contributor.authorIbacache-Quiroga, Claudia
dc.contributor.authorAssmann, Paulina
dc.contributor.authorGaggero, Aldo
dc.date.accessioned2024-09-02T16:41:14Z
dc.date.available2024-09-02T16:41:14Z
dc.date.issued2022
dc.descriptionIndexación: Scopus.
dc.description.abstractBackground: The quantification of SARS-CoV-2 in wastewater is a tool that allows determining the trend of viral circulation in a particular geographical area. Aim: To quantify the SARS-CoV-2 virus in 15 wastewater treatment plants in different Chilean cities to establish a comparison with the variables of: I) Active cases per 100,000 inhabitants; ii) daily positivity (novel cases); and iii) phases of the lockdown strategy. Methods: SARS-CoV-2 was concentrated from wastewater samples. To obtain the number of virus genomes per liter, absolute quantification was performed using qRT-PCR. Results: Between January and June 2021, 253 samples were processed, all of which were positive for the presence of the virus. Likewise, it will be determined that the rate of active cases per 100,000 inhabitants is the variable that best fits the trends obtained with the quantification of the viral load in wastewater. Conclusions: The quantification of SARS- CoV-2 in wastewater as a continuous strategy is an efficient tool to determine the trend of the viral circulation in a delimited geographical area and, combined with genomic surveillance, it can constitute an ideal sentinel surveillance alert on future outbreaks. © 2022, Sociedad Chilena de Infectologia. All rights reserved.
dc.description.urihttps://www-scielo-cl.recursosbiblioteca.unab.cl/scielo.php?script=sci_arttext&pid=S0716-10182022000600690&lng=en&nrm=iso&tlng=en
dc.identifier.citationRevista Chilena de Infectologia, Volume 39, Issue 6, Pages 690 - 698, 2022
dc.identifier.doi10.4067/S0716-10182022000600690
dc.identifier.issn0716-1018
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/59715
dc.language.isoes
dc.publisherSociedad Chilena de Infectologia
dc.rights.licenseAttribution-NonCommercial 4.0 International CC BY-NC 4.0 Deed
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/deed.en
dc.subjectActive case rate
dc.subjectGenomic surveillance
dc.subjectSARS-CoV-2
dc.subjectSentinel surveillance
dc.subjectWastewater-based epidemiology WBE
dc.titleDetection and quantification of sars-cov-2 wastewater treatment plants from different cities in chile; towards to a permanent sentinel surveillance
dc.title.alternativeDetección y cuantificación de sars-cov-2 en plantas de tratamiento de aguas residuales de diferentes ciudades de chile: Hacia la implementación de una vigilancia centinela permanente
dc.typeArtículo
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