CaracterizaciĆ³n del transcriptoma del erizo chileno (Loxechinus albus) utilizando secuenciaciĆ³n masiva
Cargando...
Archivos
Fecha
2017
Profesor/a GuĆa
Facultad/escuela
Idioma
es
TĆtulo de la revista
ISSN de la revista
TĆtulo del volumen
Editor
Universidad AndrƩs Bello
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Chile es uno de los principales exportadores de gĆ³nadas de erizo en el mundo. Sin embargo, este recurso ha sido sobreexplotado en los Ćŗltimos aƱos producto del incremento en sus tasas de captura. El erizo chileno (Loxechinus albus), se distribuye a lo largo del litoral de todo el paĆs, posee un lento crecimiento y se alimenta principalmente de algas. Este organismo no posee dimorfismo sexual externo y su forma de reproducciĆ³n es exclusivamente sexual a travĆ©s de fecundaciĆ³n externa. El erizo chileno tiene su mitogenoma secuenciado, con lo cual se estableciĆ³ la relaciĆ³n filogenĆ©tica con otros equinodermos, grupo que es ampliamente estudiado debido a su relaciĆ³n filogenĆ©tica con los cordados. Complementariamente se encuentra disponible el genoma secuenciado del erizo modelo Strongylocentrotus purpuratus, en donde se puede observar la gran similitud de genes con los vertebrados, destacando un sofisticado sistema inmune innato, siendo los celomocitos las cĆ©lulas con capacidades fagociticas que comandan su respuesta inmune. La informaciĆ³n gĆ©nica y genĆ³mica disponible sobre L. albus es muy escasa, encontrĆ”ndose solo las secuencias pertenecientes a los estudios del mitogenoma y microsatĆ©lites. Con el objetivo de āCaracterizar el transcriptoma de celomocitos, intestino y gĆ³nada del erizo chilenoā, se realizĆ³ la secuenciaciĆ³n del ARN de estos tres tejidos. Las lecturas resultantes fueron filtradas con Flexbar y ensambladas con Trinity, encontrando alrededor de ciento ochenta mil contigs, de los cuales fueron anotados el 31% de estos con la herramienta Blast. Adicionalmente, se realizĆ³ una segunda anotaciĆ³n utilizando redes de similitud de secuencia, logrando anotar un 75% de los unigenes. Con los resultados obtenidos a partir de la anotaciĆ³n y usando la herramienta DAVID, fue posible determinar los tĆ©rminos ontolĆ³gicos asociados a cada uno de los tejidos, destacando en celomocitos e intestino su rol en el sistema inmune del erizo. Por otra parte, el intestino cumplirĆa un rol clave en la homeostasis general de este organismo, y se encontrĆ³ que las gĆ³nadas serĆan un Ć³rgano con un comportamiento dual, relacionado a reproducciĆ³n y almacenamiento de nutrientes. Este trabajo sirve de base para la realizaciĆ³n de futuros estudios en esta especie, no solo para conocer la biologĆa bĆ”sica de este organismo, sino que tambiĆ©n asociado a la producciĆ³n de este recurso pesquero.
Chile is the most important producer of raw sea urchin in the world. However, this resource has been over harvested in recent years depleting their natural populations. Red sea urchin (Loxechinus albus) distributed along the Chilean coast, has a slow growth and feeds algae. Sea urchin sexual dimorphism is externally indistinguishable and has sexual reproduction through external fertilization. The mitogenome of Chilean sea urchin is sequenced, which established the phylogenetic relationship with other echinoderms. Echinoderms are widely studied due to its phylogenetic relationship with the chordates. In addition, the sequenced genome of the model sea urchin Strongylocentrotus purpuratus is available, showing a great genes similarity with vertebrates, standing out a sophisticated innate immune system, being coelomocytes cells with phagocytic capacities command immune response. The L. albus genomic information available is limited, consists in a complete mitogenome and microsatellites studies. The objective of study is "Characterize the transcriptome of coelomocytes, intestine and gonad of Chilean sea urchin", the RNA sequencing of three tissues was performed. Resulting reads were filtered with flexbar and assembled with Trinity, finding 185.239 contigs, of which 31% were annotated with Blast tool. In addition, a second annotation was made using sequence similarity networks, 75% of unigenes were identified. Results obtained from the annotation alongside with DAVID tool determined ontological terms associated to each tissue, highlighting in coelomocytes and intestine a role in the immune system. Intestine would play a key role in the homeostasis; also, we found that gonads could have a dual role in reproduction and storage of nutrients. This work provides a resource for future studies, either to increase its commercial value or study basic biology in this organism.
Chile is the most important producer of raw sea urchin in the world. However, this resource has been over harvested in recent years depleting their natural populations. Red sea urchin (Loxechinus albus) distributed along the Chilean coast, has a slow growth and feeds algae. Sea urchin sexual dimorphism is externally indistinguishable and has sexual reproduction through external fertilization. The mitogenome of Chilean sea urchin is sequenced, which established the phylogenetic relationship with other echinoderms. Echinoderms are widely studied due to its phylogenetic relationship with the chordates. In addition, the sequenced genome of the model sea urchin Strongylocentrotus purpuratus is available, showing a great genes similarity with vertebrates, standing out a sophisticated innate immune system, being coelomocytes cells with phagocytic capacities command immune response. The L. albus genomic information available is limited, consists in a complete mitogenome and microsatellites studies. The objective of study is "Characterize the transcriptome of coelomocytes, intestine and gonad of Chilean sea urchin", the RNA sequencing of three tissues was performed. Resulting reads were filtered with flexbar and assembled with Trinity, finding 185.239 contigs, of which 31% were annotated with Blast tool. In addition, a second annotation was made using sequence similarity networks, 75% of unigenes were identified. Results obtained from the annotation alongside with DAVID tool determined ontological terms associated to each tissue, highlighting in coelomocytes and intestine a role in the immune system. Intestine would play a key role in the homeostasis; also, we found that gonads could have a dual role in reproduction and storage of nutrients. This work provides a resource for future studies, either to increase its commercial value or study basic biology in this organism.
Notas
Tesis (MagĆster en BiotecnologĆa)
Palabras clave
Erizos de Mar, Transcriptoma