Neonatal mesenchymal stem cell treatment improves myelination impaired by global perinatal asphyxia in rats
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Fecha
2021-03
Profesor/a Guía
Facultad/escuela
Idioma
en
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Editor
MDPI AG
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Licencia CC
Licencia CC
Resumen
The effect of perinatal asphyxia (PA) on oligodendrocyte (OL), neuroinflammation, and cell viability was evaluated in telencephalon of rats at postnatal day (P)1, 7, and 14, a period char-acterized by a spur of neuronal networking, evaluating the effect of mesenchymal stem cell (MSCs)- treatment. The issue was investigated with a rat model of global PA, mimicking a clinical risk oc-curring under labor. PA was induced by immersing fetus-containing uterine horns into a water bath for 21 min (AS), using sibling-caesarean-delivered fetuses (CS) as controls. Two hours after delivery, AS and CS neonates were injected with either 5 μL of vehicle (10% plasma) or 5 × 104 MSCs into the lateral ventricle. Samples were assayed for myelin-basic protein (MBP) levels; Olig-1/Olig-2 tran-scriptional factors; Gglial phenotype; neuroinflammation, and delayed cell death. The main effects were observed at P7, including: (i) A decrease of MBP-immunoreactivity in external capsule, corpus callosum, cingulum, but not in fimbriae of hippocampus; (ii) an increase of Olig-1-mRNA levels; (iii) an increase of IL-6-mRNA, but not in protein levels; (iv) an increase in cell death, including OLs; and (v) MSCs treatment prevented the effect of PA on myelination, OLs number, and cell death. The present findings show that PA induces regional- and developmental-dependent changes on myelination and OLs maturation. Neonatal MSCs treatment improves survival of mature OLs and myelination in telencephalic white matter. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Indexación Scopus
Palabras clave
Remyelinization, Oligodendrocyte Precursor Cells, Cuprizone, Mesenchymal stem cells, Neonatal asphyxia/ischemia, Periventricular leukomalacia
Citación
International Journal of Molecular Sciences, Volume 22, Issue 6, 2 March 2021, Article number 3275
DOI
10.3390/ijms22063275