Neonatal mesenchymal stem cell treatment improves myelination impaired by global perinatal asphyxia in rats

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dc.contributor.authorTapia-bustos, A.
dc.contributor.authorLespay-rebolledo, C.
dc.contributor.authorVío, V.
dc.contributor.authorPérez-lobos, R.
dc.contributor.authorCasanova-ortiz, E.
dc.contributor.authorEzquer, F.
dc.contributor.authorHerrera-marschitz, M.
dc.contributor.authorMorales, P.
dc.date.accessioned2021-05-25T22:33:04Z
dc.date.available2021-05-25T22:33:04Z
dc.date.issued2021-03
dc.descriptionIndexación Scopuses
dc.description.abstractThe effect of perinatal asphyxia (PA) on oligodendrocyte (OL), neuroinflammation, and cell viability was evaluated in telencephalon of rats at postnatal day (P)1, 7, and 14, a period char-acterized by a spur of neuronal networking, evaluating the effect of mesenchymal stem cell (MSCs)- treatment. The issue was investigated with a rat model of global PA, mimicking a clinical risk oc-curring under labor. PA was induced by immersing fetus-containing uterine horns into a water bath for 21 min (AS), using sibling-caesarean-delivered fetuses (CS) as controls. Two hours after delivery, AS and CS neonates were injected with either 5 μL of vehicle (10% plasma) or 5 × 104 MSCs into the lateral ventricle. Samples were assayed for myelin-basic protein (MBP) levels; Olig-1/Olig-2 tran-scriptional factors; Gglial phenotype; neuroinflammation, and delayed cell death. The main effects were observed at P7, including: (i) A decrease of MBP-immunoreactivity in external capsule, corpus callosum, cingulum, but not in fimbriae of hippocampus; (ii) an increase of Olig-1-mRNA levels; (iii) an increase of IL-6-mRNA, but not in protein levels; (iv) an increase in cell death, including OLs; and (v) MSCs treatment prevented the effect of PA on myelination, OLs number, and cell death. The present findings show that PA induces regional- and developmental-dependent changes on myelination and OLs maturation. Neonatal MSCs treatment improves survival of mature OLs and myelination in telencephalic white matter. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.es
dc.description.urihttps://www.mdpi.com/1422-0067/22/6/3275
dc.identifier.citationInternational Journal of Molecular Sciences, Volume 22, Issue 6, 2 March 2021, Article number 3275es
dc.identifier.doi10.3390/ijms22063275
dc.identifier.issn16616596
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/18947
dc.language.isoenes
dc.publisherMDPI AGes
dc.subjectRemyelinizationes
dc.subjectOligodendrocyte Precursor Cellses
dc.subjectCuprizonees
dc.subjectMesenchymal stem cellses
dc.subjectNeonatal asphyxia/ischemiaes
dc.subjectPeriventricular leukomalaciaes
dc.titleNeonatal mesenchymal stem cell treatment improves myelination impaired by global perinatal asphyxia in ratses
dc.typeArtículoes
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