Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings

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dc.contributor.authorKato, Naohiro
dc.contributor.authorReynolds, Dexter
dc.contributor.authorBrown, Matthew L.
dc.contributor.authorBoisdore, Marietta
dc.contributor.authorFujikawa, Yukichi
dc.contributor.authorMorales, Andrea
dc.contributor.authorMeisel, Lee A.
dc.date.accessioned2024-07-30T16:39:24Z
dc.date.available2024-07-30T16:39:24Z
dc.date.issued2008-05-19
dc.descriptionINDEXACIÓN: WEB OF SCIENCE.
dc.description.abstractBackground: The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. In planta analyses of this dynamic process have typically been limited to only two organelles or proteins at a time in only a few cell types. Results: We generated a transgenic Arabidopsis plant that contains four spectrally different fluorescent proteins. Nuclei, plastids, mitochondria and plasma membranes were genetically tagged with cyan, red, yellow and green fluorescent proteins, respectively. In addition, methods to track nuclei, mitochondria and chloroplasts and quantify the interaction between these organelles at a submicron resolution were developed. These analyzes revealed that N-ethylmaleimide disrupts nuclear-mitochondrial but not nuclear-plastids interactions in root epidermal cells of live Arabidopsis seedlings. Conclusion: We developed a tool and associated methods for analyzing the complex dynamic of organelle-organelle interactions in real time in planta. Homozygous transgenic Arabidopsis (Kaleidocell) is available through Arabidopsis Biological Resource Center.
dc.description.urihttps://plantmethods.biomedcentral.com/articles/10.1186/1746-4811-4-9
dc.identifier.citationPLANT METHODS, Volume 4
dc.identifier.doi10.1186/1746-4811-4-9
dc.identifier.issn1746-4811
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/58860
dc.language.isoen
dc.publisherBMC
dc.rights.licenseCC BY 2.0 Attribution 2.0 Generic Deed
dc.rights.urihttps://creativecommons.org/licenses/by/2.0/
dc.subjectPLASMA-MEMBRANE
dc.subjectMITOCHONDRIA
dc.subjectACTIN
dc.subjectCELL
dc.subjectMICROTUBULES
dc.subjectSTROMULES
dc.subjectMOVEMENT
dc.subjectPROTEINS
dc.subjectDYNAMICS
dc.subjectPLASTIDS
dc.titleMultidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings
dc.typeArtículo
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