Interactions between Core Elements of the Botrytis cinerea Circadian Clock Are Modulated by Light and Different Protein Domains

Vista sencilla de ítem
dc.contributor.authorRojas, Vicente
dc.contributor.authorSalinas, Francisco
dc.contributor.authorRomero, Andrés
dc.contributor.authorLarrondo, Luis F.
dc.contributor.authorCanessa, Paulo
dc.date.accessioned2024-06-07T15:57:06Z
dc.date.available2024-06-07T15:57:06Z
dc.date.issued2022-05
dc.descriptionIndexación: Scopus.
dc.description.abstractBotrytis cinerea possesses a complex light-sensing system composed of eleven photoreceptors. In B. cinerea, bcwcl1 encodes for the BcWCL1 protein, the orthologue of the blue-light photoreceptor WC-1 from Neurospora crassa. The functional partner of BcWCL1 is the BcWCL2 protein, both interacting in the nucleus and forming the B. cinerea white collar complex (BcWCC). This complex is required for photomorphogenesis and circadian regulation. However, no molecular evidence shows a light-dependent interaction between the BcWCC components or light-sensing capabilities in BcWCL1. In this work, by employing a yeast two-hybrid system that allows for the in vivo analysis of protein–protein interactions, we confirm that BcWCL1 and BcWCL2 interact in the absence of light as well as upon blue-light stimulation, primarily through their PAS (Per-Arnt-Sim) domains. Deletion of the PAS domains present in BcWCL1 (BcWCL1PAS∆) or BcWCL2 (BcWCL2PAS∆) severely impairs the interaction between these proteins. Interestingly, the BcWCL1PAS∆ protein shows a blue-light response and interacts with BcWCL2 or BcWCL2PAS∆ upon light stimulation. Finally, we demonstrate that BcWCL1 and BcWCL1PAS∆ respond to blue light by introducing a point mutation in the photoactive cysteine, confirming that both proteins are capable of light sensing. Altogether, the results revealed the complexity of protein–protein interactions occurring between the core elements of the B. cinerea circadian clock. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.urihttps://www.mdpi.com/2309-608X/8/5/486
dc.identifier.citationJournal of Fungi, Volume 8, Issue 5, May 2022, Article number 486
dc.identifier.doi10.3390/jof8050486
dc.identifier.issn2309-608X
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/57412
dc.language.isoen
dc.publisherMDPI
dc.rights.licenseATRIBUCIÓN 4.0 INTERNACIONAL CC BY 4.0 Deed
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectBotrytis cinerea
dc.subjectOptogenetics
dc.subjectPAS and LOV domains
dc.subjectPhotoreceptors
dc.subjectYeast
dc.titleInteractions between Core Elements of the Botrytis cinerea Circadian Clock Are Modulated by Light and Different Protein Domains
dc.typeArtículo
Archivos
Bloque original
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
Rojas_Interactions_between_core_elements_of.pdf
Tamaño:
1.01 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLÉS
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista