Identification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublin

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dc.contributor.authorAmaya, F.
dc.contributor.authorBlondel, C.
dc.contributor.authorBarros-Infante, M.
dc.contributor.authorRivera, D.
dc.contributor.authorMoreno-Switt, A.
dc.contributor.authorSantiviago, C.
dc.contributor.authorPezoa, D.
dc.date.accessioned2022-08-02T17:16:58Z
dc.date.available2022-08-02T17:16:58Z
dc.date.issued2022-02
dc.descriptionIndexación: Scopus.es
dc.description.abstractThe Type VI Secretion System (T6SS) is a multiprotein device that has emerged as an important fitness and virulence factor for many Gram-negative bacteria through the injection of effector proteins into prokaryotic or eukaryotic cells via a contractile mechanism. While some effector proteins specifically target bacterial or eukaryotic cells, others can target both types of cells (trans-kingdom effectors). In Salmonella, five T6SS gene clusters have been identified within pathogenicity islands SPI-6, SPI-19, SPI-20, SPI-21, and SPI-22, which are differentially distributed among serotypes. Salmonella enterica serotype Dublin (S. Dublin) is a cattle-adapted pathogen that harbors both T6SSSPI-6 and T6SSSPI-19. Interestingly, while both systems have been linked to virulence and host colonization in S. Dublin, an antibacterial activity has not been detected for T6SSSPI-6 in this serotype. In addition, there is limited information regarding the repertoire of effector proteins encoded within T6SSSPI-6 and T6SSSPI-19 gene clusters in S. Dublin. In the present study, we demonstrate that T6SSSPI-6 and T6SSSPI-19 of S. Dublin CT_02021853 contribute to interbacterial competition. Bioinformatic and comparative genomic analyses allowed us to identify genes encoding three candidate antibacterial effectors located within SPI-6 and two candidate effectors located within SPI-19. Each antibacterial effector gene is located upstream of a gene encoding a hypothetic immunity protein, thus conforming an effector/immunity (E/I) module. Of note, the genes encoding these effectors and immunity proteins are widely distributed in Salmonella genomes, suggesting a relevant role in interbacterial competition and virulence. Finally, we demonstrate that E/I modules SED_RS01930/SED_RS01935 (encoded in SPI-6), SED_RS06235/SED_RS06230, and SED_RS06335/SED_RS06340 (both encoded in SPI-19) contribute to interbacterial competition in S. Dublin CT_02021853.es
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2022.811932/full
dc.identifier.citationFrontiers in Microbiology, Volume 1310, February 2022, Article number 811932es
dc.identifier.doi10.3389/fmicb.2022.811932
dc.identifier.issn1664-302X
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/23410
dc.language.isoenes
dc.publisherFrontiers Media S.A.es
dc.rights.licenseAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://www.frontiersin.org/about/open-access/
dc.subjecteffectores
dc.subjectimmunity proteines
dc.subjectinterbacterial competitiones
dc.subjectSalmonella Dublines
dc.subjectT6SSes
dc.subjectBacterial Proteinses
dc.subjectPyocinses
dc.subjectVibrio Choleraees
dc.titleIdentification of Type VI Secretion Systems Effector Proteins That Contribute to Interbacterial Competition in Salmonella Dublines
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