Microwave-Assisted Extraction of Secondary Metabolites Using Ethyl Lactate Green Solvent from Ambrosia arborescens: LC/ESI-MS/MS and Antioxidant Activity

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dc.contributor.authorGuillen, Evelyn
dc.contributor.authorTerrones, Hector
dc.contributor.authorde Terrones, Teresa Cano
dc.contributor.authorSimirgiotis, Mario J.
dc.contributor.authorHájek, Jan
dc.contributor.authorCheel, José
dc.contributor.authorSepulveda, Beatriz
dc.contributor.authorAreche, Carlos
dc.date.accessioned2024-08-14T15:47:16Z
dc.date.available2024-08-14T15:47:16Z
dc.date.issued2024-05
dc.descriptionIndexación: Scopus.
dc.description.abstractAlternative solvents are being tested as green solvents to replace the traditional organic solvents used in both academy and industry. Some of these are already available, such as ethyl lactate, cyrene, limonene, glycerol, and others. This alternative explores eco-friendly processes for extracting secondary metabolites from nature, thus increasing the number of unconventional extraction methods with lower environmental impact over conventional methods. In this context, the Peruvian Ambrosia arborescens was our model while exploring a microwave-assisted extraction (MAE) approach over maceration. The objective of this study was to perform a phytochemical study including UHPLC-ESI-MS/MS and the antioxidant activity of Ambrosia arborescens, using sustainable strategies by mixing both microwaves and ethyl lactate as a green solvent. The results showed that ethyl lactate/MAE (15.07%) achieved a higher extraction yield than methanol/maceration (12.6%). In the case of the isolation of psilostachyin, it was similar to ethyl lactate (0.44%) when compared to methanol (0.40%). Regarding UHPLC-ESI-MS/MS studies, the results were similar. Twenty-eight compounds were identified in the ethyl lactate/MAE and methanol/maceration extracts, except for the tentative identification of two additional amino acids (peaks 4 and 6) in the MeOH extract. In relation to the antioxidant assay, the activity of the ethyl lactate extract was a little higher than the methanol extract in terms of ORAC (715.38 ± 3.2) and DPPH (263.04 ± 2.8). This study on A. arborescens demonstrated that the unconventional techniques, such as MAE related to ethyl lactate, could replace maceration/MeOH for the extraction and isolation of metabolites from diverse sources. This finding showed the potential of unconventional methods with green solvents to provide eco-friendly methods based on green chemistry.
dc.description.urihttps://www-scopus-com.recursosbiblioteca.unab.cl/record/display.uri?eid=2-s2.0-85192687356&origin=resultslist&sort=plf-f&src=s&sid=688d84fa765aa76ed089413b3e00c4fb&sot=aff&sdt=cl&cluster=scosubjabbr%2C%22AGRI%22%2Ct%2Bscosubtype%2C%22ar%22%2Ct%2Bscofreetoread%2C%22all%22%2Ct&s=AF-ID%2860002636%29+AND+SUBJAREA%28ENVI%29&sl=34&sessionSearchId=688d84fa765aa76ed089413b3e00c4fb&relpos=2
dc.identifier.citationPlants Open Access Volume 13, Issue 9 May 2024 Article number 1213
dc.identifier.doi10.3390/plants13091213
dc.identifier.issn22237747
dc.identifier.urihttps://repositorio.unab.cl/handle/ria/59252
dc.language.isoen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.rights.licenseCC BY 4.0 Attribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectalternative solvents
dc.subjectAmbrosia arborescens
dc.subjectethyl lactate
dc.subjectgreen extraction
dc.subjectmicrowave
dc.subjectnon-conventional technology
dc.titleMicrowave-Assisted Extraction of Secondary Metabolites Using Ethyl Lactate Green Solvent from Ambrosia arborescens: LC/ESI-MS/MS and Antioxidant Activity
dc.typeArtículo
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