Interleukin-4 Selectively Inhibits Interleukin-2 Secretion by Lipopolysaccharide-Activated Dendritic Cells
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Archivos
Fecha
2004-02
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Wiley
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Dendritic cells (DCs) generated in vitro from bone marrow precursors using granulocyte-macrophage colony-stimulating factor (GM-CSF) secrete interleukin-2 (IL-2) upon activation, an event probably associated to the initiation of adaptive immune responses. Additionally, they produce IL-12, a cytokine related to T-cell polarization. To analyse the effect of IL-4 on DC differentiation and function, we assessed the capacity of murine bone marrow dendritic cells (BMDCs) differentiated with GM-CSF in the presence or absence of IL-4 to produce IL-2 and IL-12 upon lipopolysaccharide (LPS) activation. We found that although IL-4 enhanced DC IL-12p70 production, it strongly impaired IL-2 secretion by BMDCs. This inhibition, which depends on the presence of IL-4 during LPS activation, is DC specific, as IL-4 did not affect IL-2 secretion by T cells. Interestingly, inhibition of DC IL-2 production did not prevent DC priming of T lymphocytes. These results illustrate a new putative role for IL-4 on the regulation of the immune response and should help clarify the controversial reports on the effect of IL-4 on DCs.
Notas
Indexación: Scopus.
Palabras clave
Natural Killer Cells, Cell Differentiation, Coculture Techniques, Dendritic Cells, Flow Cytometry, Interleukin-12, Interleukin-2, Interleukin-4, Lipopolysaccharides, Lymphocyte Activation
Citación
Scandinavian Journal of Immunology, Volume 59, Issue 2, Pages 183 - 189February 2004
DOI
DOI: 10.1111/j.0300-9475.2004.01380.x