Using a ligate intestinal loop mouse model to investigate Clostridioides difficile adherence to the intestinal mucosa in aged mice

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dc.contributor.authorCastro-Córdova, Pablo
dc.contributor.authorMendoza-León, María José
dc.contributor.authorParedes-Sabja, Daniel
dc.date.accessioned2023-12-07T14:53:23Z
dc.date.available2023-12-07T14:53:23Z
dc.date.issued2021-12
dc.descriptionIndexación (Scopus)es
dc.description.abstractInteraction of Clostridioides difficile spores with the intestinal mucosa contributes to the persistence and recurrence of the infection. Advanced age is one of the main risk factors for C. difficile infection and recurrence of the disease. However, interaction of C. difficile spores with the intestinal mucosa during aging has not been evaluated. In the present work, using intestinal ligated loop technique in a mouse model, we analyzed C. difficile spore adherence and internalization to the ileum and colonic mucosa during aging. Additionally, we provide visual documentation of the critical steps of the procedure. Consequently, our data suggest that spore internalization in the ileum and colonic mucosa is higher in elderly mice rather than adults or young mice. Also, our data suggest that spore adherence to the ileum and colonic mucosa decreases with aging.es
dc.identifier.citationPLoS ONE Volume 16, Issue 12 December December 2021 Article number e0261081es
dc.identifier.doi10.1371/journal.pone.0261081en
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/54486
dc.language.isoenes
dc.publisherPublic Library of Sciencees
dc.rights.licenseCC BY 4.0 Deed Atribución 4.0 Internacionalen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.esen
dc.subjectAginges
dc.subjectAnimalses
dc.subjectAttachment Siteses
dc.subjectMicrobiologicales
dc.subjectBacterial Adhesiones
dc.subjectClostridioides difficilees
dc.subjectClostridium Infectionses
dc.subjectDisease Models, Animales
dc.subjectFemalees
dc.subjectIntestinal Mucosaes
dc.subjectMalees
dc.subjectMicees
dc.subjectMice Inbred C57BLes
dc.subjectMicroscopy, Confocales
dc.subjectSpores, Bacteriales
dc.titleUsing a ligate intestinal loop mouse model to investigate Clostridioides difficile adherence to the intestinal mucosa in aged micees
dc.typeArtículoes
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