Aislamiento y caracterización del gen que codifica para la proteína ribosomal L41 del pez Cyprinus carpio
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2004
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es
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Universidad Andrés Bello
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Licencia CC
Licencia CC
Resumen
La temperatura corporal de los peces está directamente relacionada con la de su hábitat natural, junto al fotoperíodo, es la variable ambiental más importante que influye en su desarrollo y crecimiento. Trabajos anteriores han reportado que durante la aclimatización estacional ocurren profundos cambios ultraestructurales en el nucléolo de células provenientes de diferentes tejidos del pez Cyprinus carpio. Asimismo, se observó una depresión de la síntesis de rRNAs y tRNAs en invierno, reflejo de una reprogramación fisiológica y bioquímica que implica importantes cambios en la expresión génica indispensables para la adaptación de este pez a los cambios físicos de su entorno.
Nosotros utilizamos la proteína ribosomal L41 como modelo para estudiar los mecanismos que controlan la síntesis de ribosomas en relación con el proceso de aclimatización de la carpa.
De esta forma rastreamos el gen que codifica para la proteína ribosomal L41 desde una biblioteca genómica de carpa utilizando un sonda específica. Aislamos dos clones positivos. A partir de uno de ellos, obtuvimos la secuencia completa del gen y caracterizamos su estructura. Asimismo, estudiamos la expresión estacional del mRNA del gen L41 en diferentes tejidos de la carpa. Nuestros hallazgos muestran que el gen L41 se transcribe en mayores niveles en carpas aclimatizadas a verano respecto de carpas aclimatizadas a invierno.
The body temperatura of fish is directly related with their natural environment, and together with the photo-period, is the most important parameter which influences their development and growth. Previous studies have reported that during seasonal acclimatization important ultrastructural changes occur in the nucleolus of cells from different tissues of the Cyprinus carpio fish. Furthermore, a down-regulation of rRNA and tRNA synthesis has been observed in winter, indicating physiological and biochemical reprogramming which implies important changes in gene expression vital for the adaptation of this fish to physical changes in its environment. We used the ribosomal protein L41 as a model to study the mechanisms controling ribosome syntesis in relation with the acclimatization process of the carp. We screened a carp genomic library for the gene encoding for the ribosomal protein L41 using an homologous probe. We isolated two positive clones and, from one of these, we obtained the complete gene sequence and characterized its structure. Furthermore, we also studied the seasanal expression of the carp L41 mRNA in different tissues. Our findings show that the L41 gene is transcribed in higher levels in carps acclimatized to summer comparing to those acclimatized to winter.
The body temperatura of fish is directly related with their natural environment, and together with the photo-period, is the most important parameter which influences their development and growth. Previous studies have reported that during seasonal acclimatization important ultrastructural changes occur in the nucleolus of cells from different tissues of the Cyprinus carpio fish. Furthermore, a down-regulation of rRNA and tRNA synthesis has been observed in winter, indicating physiological and biochemical reprogramming which implies important changes in gene expression vital for the adaptation of this fish to physical changes in its environment. We used the ribosomal protein L41 as a model to study the mechanisms controling ribosome syntesis in relation with the acclimatization process of the carp. We screened a carp genomic library for the gene encoding for the ribosomal protein L41 using an homologous probe. We isolated two positive clones and, from one of these, we obtained the complete gene sequence and characterized its structure. Furthermore, we also studied the seasanal expression of the carp L41 mRNA in different tissues. Our findings show that the L41 gene is transcribed in higher levels in carps acclimatized to summer comparing to those acclimatized to winter.
Notas
Tesis (Ingeniería en Acuicultura)
Palabras clave
Proteína ribosomal L41, Cyprinus carpio, Aclimatización estacional, Investigaciones genéticas, Peces