Caries-free subjects have high levels of urease and arginine deiminase activity

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dc.contributor.authorReyes, Evelyn
dc.contributor.authorMartin, Javier
dc.contributor.authorMoncada, Gustavo
dc.contributor.authorNeira, Miguel
dc.contributor.authorPalma, Patricia
dc.contributor.authorGordan, Valeria
dc.contributor.authorOyarzo, Juan Fernando
dc.contributor.authorYévenes, Ismael
dc.date.accessioned2016-07-04T16:22:24Z
dc.date.available2016-07-04T16:22:24Z
dc.date.issued2014
dc.descriptionIndexación: Web of Science; Scielo.es
dc.description.abstractObjectives: This study investigated the relationship between urease and arginine deiminase system (ADS) activities and dental caries through a cross-sectional study. Material and Methods: Urease and ADS activities were measured in saliva and plaque samples from 10 caries-free subjects and 13 caries-active. Urease activity was obtained from the ammonia produced by incubation of plaque and saliva samples in urea. ADS activity was obtained from the ammonia generated by the arginine-HCl and Tris-maleate buffer. Specific activity was defined as micromoles of ammonia per minute per milligram of protein. Shapiro-Wilk statistical test was used to analyze the distribution of the data, and Mann-Whitney test was used to determine the significance of the data. Results: The specific urease activity in saliva and plaque was significantly higher in individuals with low DMFT scores. ADS activity in saliva (6.050 vs 1.350, p=0.0154) and plaque (8.830 vs 1.210, p=0.025) was also higher in individuals with low DMFT scores. Conclusions: Caries-free subjects had a higher ammonia generation activity by urease and arginine deiminase system for both saliva and plaque samples than low caries-active subjects. High levels of alkali production in oral environment were related to caries-free subjects.es
dc.description.urihttp://ref.scielo.org/ch4qbr
dc.identifier.citationJ. Appl. Oral Sci. vol.22 no.3 Bauru May./June 2014es
dc.identifier.issn1678-7757
dc.identifier.otherhttp://dx.doi.org/10.1590/1678-775720130591
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/947
dc.language.isoenes
dc.publisherFaculdade De Odontologia De Bauru - USPes
dc.subjectAlkalies
dc.subjectArgininees
dc.subjectDental plaquees
dc.subjectSalivaes
dc.subjectUreasees
dc.subjectDental carieses
dc.titleCaries-free subjects have high levels of urease and arginine deiminase activityes
dc.typeArtículoes
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