Estudio del efecto de la fuente de azúcar sobre la motilidad de roseburia inulinivorans y su relación con la formación de biopelícula
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2023
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es
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Universidad Andrés Bello
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Licencia CC
Licencia CC
Resumen
Roseburia es un género bacteriano conformado por cinco especies: Roseburia
intestinalis, Roseburia hominis, Roseburia inulinivorans, Roseburia faecis y
Roseburia cecicola, las cuales tienen la capacidad de colonizar el tracto
gastrointestinal de los seres humanos y producir ácidos grasos de cadena corta
(AGCC) otorgando energía y propiedades antiinflamatorias al colon, siendo
parte de la gran diversidad bacteriana que compone la microbiota intestinal.
La microbiota intestinal es un ecosistema microbiano que habita en el tracto
intestinal y forma una especie de simbiosis en la que las bacterias viven en el
intestino otorgando propiedades que optimizan la homeostasis de nuestro
organismo. Esta relación simbiótica es de suma importancia para la salud,
incidiendo en el tránsito intestinal, correcto funcionamiento del colon, etc.
Es así, como a lo largo de la historia, han ido aumentando la cantidad de
estudios que permitan conocer la diversidad microbiana del intestino, para
analizar su composición, funcionamiento y beneficios, además de poder
comprender la forma en la que estas bacterias colonizan el intestino y lograr
encontrar métodos que las resguarden de agentes antimicrobianos como los
antibióticos, que al ingerirlos afectan negativamente a estas bacterias
comensales, y a su vez, a nuestro sistema inmune.
En este estudio se analizó el comportamiento fenotípico de cuatro cepas
distintas de Roseburia inulinivorans en base a la capacidad de motilidad que
posee cada cepa en distintas concentraciones de azúcares del medio. Se cree
que la fermentación de azúcares tiene relación con la formación de butirato, un
ácido graso involucrado en la obtención de ATP, lo que afectaría procesos
celulares en Roseburia, además, se ha visto en otros ejemplares que la fuente
de carbono afecta funciones como la motilidad. Posterior a esto, se estudió la
capacidad formadora de biopelículas de cada cepa, contrastando los resultados
obtenidos y evaluando si existe una relación entre la pérdida de motilidad y la
formación de biopelículas.
Roseburia is a bacterial genus made up of five species: Roseburia intestinalis, Roseburia hominis, Roseburia inulinivorans, Roseburia faecis and Roseburia cecicola, which have the ability to colonize the gastrointestinal tract of humans and produce short chain fatty acids (SCFA) providing energy and antiinflammatory properties to the colon, being part of the great bacterial diversity that makes up the intestinal microbiota. The intestinal microbiota is a microbial ecosystem that inhabits the intestinal tract and forms a kind of symbiosis in which bacteria live in the intestine providing properties that optimize the homeostasis of our organism. This symbiotic relationship is of utmost importance for health, affecting intestinal transit, correct functioning of the colon, etc. Thus, throughout history, there has been an increase in the number of studies that allow us to know the microbial diversity of the intestine, to analyze its composition, functioning and benefits, as well as to understand the way in which these bacteria colonize the intestine and to find methods to protect them from antimicrobial agents such as antibiotics, which when ingested negatively affect these commensal bacteria, and in turn, our immune system. In this study, the phenotypic behavior of four different strains of Roseburia inulinivorans was analized based on the motility capacity of each strain in different concentrations of sugars in the medium. It is believed that the fermentation of sugars is related to the formation of butyrate, a fatty acid involved in obtaining ATP, which would affect celular processes in Roseburia, in addition, it has been seen in other specimens that the carbon source affects functions such as motiliy. Subsequently, the biofilm-forming capacity of each strain was studied, contrasting the results obtained and evaluating whether there is a relationship between the loss of motility and biofilm formation.
Roseburia is a bacterial genus made up of five species: Roseburia intestinalis, Roseburia hominis, Roseburia inulinivorans, Roseburia faecis and Roseburia cecicola, which have the ability to colonize the gastrointestinal tract of humans and produce short chain fatty acids (SCFA) providing energy and antiinflammatory properties to the colon, being part of the great bacterial diversity that makes up the intestinal microbiota. The intestinal microbiota is a microbial ecosystem that inhabits the intestinal tract and forms a kind of symbiosis in which bacteria live in the intestine providing properties that optimize the homeostasis of our organism. This symbiotic relationship is of utmost importance for health, affecting intestinal transit, correct functioning of the colon, etc. Thus, throughout history, there has been an increase in the number of studies that allow us to know the microbial diversity of the intestine, to analyze its composition, functioning and benefits, as well as to understand the way in which these bacteria colonize the intestine and to find methods to protect them from antimicrobial agents such as antibiotics, which when ingested negatively affect these commensal bacteria, and in turn, our immune system. In this study, the phenotypic behavior of four different strains of Roseburia inulinivorans was analized based on the motility capacity of each strain in different concentrations of sugars in the medium. It is believed that the fermentation of sugars is related to the formation of butyrate, a fatty acid involved in obtaining ATP, which would affect celular processes in Roseburia, in addition, it has been seen in other specimens that the carbon source affects functions such as motiliy. Subsequently, the biofilm-forming capacity of each strain was studied, contrasting the results obtained and evaluating whether there is a relationship between the loss of motility and biofilm formation.
Notas
Tesis (Licenciada en Biología)
Palabras clave
Roseburia, Microbiota Intestinal, Motilidad Gastrointestinal