The small RNA RyhB homologs from Salmonella typhimurium participate in the response to S-nitrosoglutathione-induced stress
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Fecha
2014-07
Profesor/a Guía
Facultad/escuela
Idioma
en
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Editor
Academic Press Inc.
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Licencia CC
Atribution 4.0 International (CC BY 4.0)
Licencia CC
https://creativecommons.org/licenses/by/4.0/deed.es
Resumen
Typically, the expression of sRNAs is activated in response to environmental stimuli in order to regulate
gene expression through post-transcriptional mechanisms. In the present work we show that the Salmo nella typhimurium paralogous sRNAs RyhB-1 and RyhB-2 are induced in response to the nitrosating agent
S-nitrosoglutathione (GSNO). Inactivation of these sRNAs decreased S. typhimurium resistance to GSNO
and increased the levels of nitrosylated proteins. These results prompted us to evaluate a possible role
of these sRNAs in nitrosative stress resistance. RNA profiling was used as a screen to identify novel
RyhB-1 and RyhB-2 regulated targets. A subset of genes was filtered based on their potential role in
the response to nitrosative stress and their expression was analyzed by quantitative RT-PCR in wild type,
single and double mutant strains (DryhB1, DryhB2 and DryhB1 DryhB2) treated with GSNO. In response to
GSNO RyhB-1 and RyhB-2 negatively regulate the expression of the genes cyoABC (cytochrome bo oxi dase), cydB (cytochrome bd oxidase), cybC (cytochrome b-562), and positively regulate the nirBCD operon
(nitrite reductase system). Together, these results suggest that RyhB-1 and RyhB-2 finely tune the expres sion of genes coding for cytochrome oxidases and the nitrate reductase system, allowing the cell to cope
with GSNO-induced stress.
2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND
license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
Notas
Indexación: Scopus.
Palabras clave
sRNA, RyhB, Nitrosative Stress, GSNO, RNS
Citación
Biochemical and Biophysical Research Communications. Volume 450, Issue 1, Pages 641 - 645. 18 July 2014
DOI
10.1016/j.bbrc.2014.06.031