The small RNA RyhB homologs from Salmonella typhimurium participate in the response to S-nitrosoglutathione-induced stress

Vista sencilla de ítem
dc.contributor.authorCalderón, Paulina F.
dc.contributor.authorMorales, Eduardo H.
dc.contributor.authorAcuña, Lillian G.
dc.contributor.authorFuentes, Danitza N.
dc.contributor.authorGil, Fernando
dc.contributor.authorPorwollik, S.
dc.contributor.authorMcClelland, Michael
dc.contributor.authorSaavedra, Claudia P.
dc.contributor.authorCalderón, Iván L.
dc.date.accessioned2023-06-02T20:39:18Z
dc.date.available2023-06-02T20:39:18Z
dc.date.issued2014-07
dc.descriptionIndexación: Scopus.es
dc.description.abstractTypically, the expression of sRNAs is activated in response to environmental stimuli in order to regulate gene expression through post-transcriptional mechanisms. In the present work we show that the Salmo nella typhimurium paralogous sRNAs RyhB-1 and RyhB-2 are induced in response to the nitrosating agent S-nitrosoglutathione (GSNO). Inactivation of these sRNAs decreased S. typhimurium resistance to GSNO and increased the levels of nitrosylated proteins. These results prompted us to evaluate a possible role of these sRNAs in nitrosative stress resistance. RNA profiling was used as a screen to identify novel RyhB-1 and RyhB-2 regulated targets. A subset of genes was filtered based on their potential role in the response to nitrosative stress and their expression was analyzed by quantitative RT-PCR in wild type, single and double mutant strains (DryhB1, DryhB2 and DryhB1 DryhB2) treated with GSNO. In response to GSNO RyhB-1 and RyhB-2 negatively regulate the expression of the genes cyoABC (cytochrome bo oxi dase), cydB (cytochrome bd oxidase), cybC (cytochrome b-562), and positively regulate the nirBCD operon (nitrite reductase system). Together, these results suggest that RyhB-1 and RyhB-2 finely tune the expres sion of genes coding for cytochrome oxidases and the nitrate reductase system, allowing the cell to cope with GSNO-induced stress. 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).es
dc.description.urihttps://www-sciencedirect-com.recursosbiblioteca.unab.cl/science/article/pii/S0006291X1401105X?via%3Dihub
dc.identifier.citationBiochemical and Biophysical Research Communications. Volume 450, Issue 1, Pages 641 - 645. 18 July 2014es
dc.identifier.doi10.1016/j.bbrc.2014.06.031
dc.identifier.issn0006-291X
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/50299
dc.language.isoenes
dc.publisherAcademic Press Inc.es
dc.rights.licenseAtribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectsRNAes
dc.subjectRyhBes
dc.subjectNitrosative Stresses
dc.subjectGSNOes
dc.subjectRNSes
dc.titleThe small RNA RyhB homologs from Salmonella typhimurium participate in the response to S-nitrosoglutathione-induced stresses
dc.typeArtículoes
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Calderon_The_small_RNA_RyhB_homologs_from_Salmonella_typhimurium_participate.pdf
Tamaño:
664.33 KB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLÉS
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
Fac.CV - Artículos de Revista