Fast detection of phenolic compounds in extracts of easter pears (pyrus communis) from the atacama desert by ultrahigh-performance liquid chromatography and mass spectrometry (UHPLC-Q/Orbitrap/MS/MS)

A small Chilean variety of pears growing in the town of Toconao, an oasis located at the northeastern edge of the Salar de Atacama, northern Chile, was studied by means of modern PDA and high resolution mass spectral data (UHPLC-PDA-HESI-orbitrap-MS/MS). In addition, the antioxidant features of the fruits were compared with the varieties Packhman’s Triumph and Abate Fetel and correlated with the presence of phenolic compounds. The non-pigmented phenolics were fingerprinted and related to the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics and flavonoids measured by spectroscopic methods. The machine allowed a fast separation of 15 min employing a flow rate of 1 mL per minute and could accurately identify 25 compounds, including several isorhamnetin derivatives and phenolic acids, present in the peel and pulps of this Chilean variety for the first time. The compounds were monitored using a wavelength range of 210–800 nm. The native small Chilean pear showed the highest antioxidant activity measured as the bleaching of the DPPH radical, the ferric reducing antioxidant power and superoxide anion scavenging activity (8.61 ˘ 0.65 µg/mL, 712.63 ˘ 12.12 micromols trolox equivalents (µmol/TE)/100 g FW, and 82.89% ˘ 2.52% at 100 µg/mL, respectively).

Notas

Indexación: Scopus.

Palabras clave

Chilean Pears, Pera de Pascua, Phenolics, Antioxidant Capacity, UHPLC-MS, Orbitrap (OT), Proanthocyanidins, Flavonoids

Citación

Molecules. Volume 21, Issue 1. 1 January 2016. Article number 21010092

DOI

10.3390/molecules21010092

URI

https://repositorio.unab.cl/xmlui/handle/ria/54316

Colecciones

FCE - Artículos de Revista

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