NIR and glutathione trigger the surface release of methotrexate linked by Diels-Alder adducts to anisotropic gold nanoparticles

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dc.contributor.authorBolaños, Karen
dc.contributor.authorSánchez-Navarro, Macarena
dc.contributor.authorGiralt, Ernest
dc.contributor.authorAcosta, Gerardo
dc.contributor.authorAlbericio, Fernando
dc.contributor.authorKogan, Marcelo J.
dc.contributor.authorAraya, Eyleen
dc.date.accessioned2023-11-02T14:46:51Z
dc.date.available2023-11-02T14:46:51Z
dc.date.issued2021-12
dc.descriptionIndexación: Scopuses
dc.description.abstractThe administration and controlled release of drugs over time remains one of the greatest challenges of science today. In the nanomaterials field, anisotropic gold nanoparticles (AuNPs) with plasmon bands centered at the near-infrared region (NIR), such as gold nanorods (AuNRs) and gold nanoprisms (AuNPrs), under laser irradiation, locally increase the temperature, allowing the release of drugs. In this sense, temporally controlled drug delivery could be promoted by external stimuli using thermo-reversible chemical reactions, such as Diels-Alder cycloadditions from a diene and a dienophile fragment (compound a). In this study, an antitumor drug (methotrexate, MTX) was linked to plasmonic AuNPs by a Diels-Alder adduct (compound c), which after NIR suffers a retro-Diels-Alder reaction, producing release of the drug (compound b). We obtained two nanosystems based on AuNRs and AuNPrs. Both nanoconstructs were coated with BSA-r8 (Bovine Serum Albumin functionalized with Arg8, all-D octa arginine) in order to increase the colloidal stability and promote internalization of the nanosystems on HeLa and SK-BR-3 cells. In addition, the presence of BSA allows protecting the cargo from being released on the extracellular environment and promotes the photothermal release of the drug in the presence of glutathione (GSH). The nanosystems' drug release profile was evaluated after NIR irradiation in the presence and absence of glutathione (GSH), showing a considerable increase of drug release when NIR light and glutathione were combined. This work broadens the range of possibilities of using two complementary strategies for the controlled release of an antitumor drug from AuNRs and AuNPrs: the photothermal cleavage of a thermolabile adduct controlled by an external stimulus (laser irradiation), complemented with the use of the intracellular metabolite GSH.es
dc.identifier.citationMaterials Science and Engineering C Volume 1 31December 2021 Article number 112512es
dc.identifier.doi10.1016/j.msec.2021.112512en
dc.identifier.issn0928-4931
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/53800
dc.language.isoenes
dc.publisherElsevier Ltdes
dc.rights.licenseCC BY-ND 4.0 DEED Attribution-NoDerivs 4.0 Internationalen
dc.rights.urihttps://creativecommons.org/licenses/by-nd/4.0/?&en
dc.subjectDiels-Alder adductses
dc.subjectGlutathionees
dc.subjectGold nanoprismes
dc.subjectGold nanorodses
dc.subjectLaser irradiationes
dc.subjectMethotrexatees
dc.titleNIR and glutathione trigger the surface release of methotrexate linked by Diels-Alder adducts to anisotropic gold nanoparticleses
dc.typeArtículoes
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