Dynamic distribution of hig2a between the mitochondria and the nucleus in response to hypoxia and oxidative stress

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dc.contributor.authorCelia Salazar
dc.contributor.authorMiriam Barros
dc.contributor.authorAlvaro A. Elorza
dc.contributor.authorLina María Ruiz
dc.date.accessioned2022-10-11T23:39:37Z
dc.date.available2022-10-11T23:39:37Z
dc.date.issued2022-01
dc.description.abstractMitochondrial respiratory supercomplex formation requires HIG2A protein, which also has been associated with cell proliferation and cell survival under hypoxia. HIG2A protein localizes in mitochondria and nucleus. DNA methylation and mRNA expression of the HIGD2A gene show significant alterations in several cancers, suggesting a role for HIG2A in cancer biology. The present work aims to understand the dynamics of the HIG2A subcellular localization under cellular stress. We found that HIG2A protein levels increase under oxidative stress. H2O2 shifts HIG2A localization to the mitochondria, while rotenone shifts it to the nucleus. HIG2A protein colocalized at a higher level in the nucleus concerning the mitochondrial network under normoxia and hypoxia (2% O2 ). Hypoxia (2% O2 ) significantly increases HIG2A nuclear colocalization in C2C12 cells. In HEK293 cells, chemical hypoxia with CoCl2 (>1% O2 ) and FCCP mitochondrial uncoupling, the HIG2A protein decreased its nuclear localization and shifted to the mitochondria. This suggests that the HIG2A distribution pattern between the mitochondria and the nucleus depends on stress and cell type. HIG2A protein expression levels increase under cellular stresses such as hypoxia and oxidative stress. Its dynamic distribution between mitochondria and the nucleus in response to stress factors suggests a new communication system between the mitochondria and the nucleus. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).es
dc.description.sponsorshipIndexación: Scopuses
dc.identifier.citationInternational Journal of Molecular Sciences Open AccessVolume 23, Issue 1January-1 2022 Article number 389es
dc.identifier.issn16616596
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/24235
dc.language.isoenes
dc.publisherMDPIes
dc.subjectCancer; HIGD2A; Hypoxia; Metabolic reprograming; Mitochondria; Oxidative stresses
dc.titleDynamic distribution of hig2a between the mitochondria and the nucleus in response to hypoxia and oxidative stresses
dc.typeArtículoes
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