Ezh2-dependent H3K27me3 modification dynamically regulates vitamin D3-dependent epigenetic control of CYP24A1 gene expression in osteoblastic cells

Vista sencilla de ítem
dc.contributor.authorMoena, D.
dc.contributor.authorNardocci, G.
dc.contributor.authorAcevedo, E.
dc.contributor.authorLian, J.
dc.contributor.authorStein, G.
dc.contributor.authorStein, J.
dc.contributor.authorMontecino, M.
dc.date.accessioned2021-11-19T18:03:06Z
dc.date.available2021-11-19T18:03:06Z
dc.date.issued2020-01
dc.descriptionIndexación: Scopuses
dc.description.abstractEpigenetic control is critical for the regulation of gene transcription in mammalian cells. Among the most important epigenetic mechanisms are those associated with posttranslational modifications of chromosomal histone proteins, which modulate chromatin structure and increased accessibility of promoter regulatory elements for competency to support transcription. A critical histone mark is trimethylation of histone H3 at lysine residue 27 (H3K27me3), which is mediated by Ezh2, the catalytic subunit of the polycomb group complex PRC2 to repress transcription. Treatment of cells with the active vitamin D metabolite 1,25(OH)2D3, results in transcriptional activation of the CYP24A1 gene, which encodes a 24-hydroxylase enzyme, that is, essential for physiological control of vitamin D3 levels. We report that the Ezh2-mediated deposition of H3K27me3 at the CYP24A1 gene promoter is a requisite regulatory component during transcriptional silencing of this gene in osteoblastic cells in the absence of 1,25(OH)2D3. 1,25(OH)2D3 dependent transcriptional activation of the CYP24A1 gene is accompanied by a rapid release of Ezh2 from the promoter, together with the binding of the H3K27me3-specific demethylase Utx/Kdm6a and thereby subsequent erasing of the H3K27me3 mark. Importantly, we find that these changes in H3K27me3 enrichment at the CYP24A1 gene promoter are highly dynamic, as this modification is rapidly reacquired following the withdrawal of 1,25(OH)2D3. © 2020 Wiley Periodicals, Inc.es
dc.description.urihttps://onlinelibrary-wiley-com.recursosbiblioteca.unab.cl/doi/10.1002/jcp.29428
dc.identifier.citationJournal of Cellular Physiology Volume 235, Issue 6, Pages 5404 - 54121 June 2020es
dc.identifier.doi10.1002/jcp.29428
dc.identifier.issn0021-9541
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/20983
dc.language.isoenes
dc.publisherWiley-Liss Inc.es
dc.subjectEpigenetic control of transcriptiones
dc.subjectOsteoblast gene transcription.es
dc.subjectVitamin D-mediated transcription.es
dc.titleEzh2-dependent H3K27me3 modification dynamically regulates vitamin D3-dependent epigenetic control of CYP24A1 gene expression in osteoblastic cellses
dc.typeArtículoes
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Moena_D_Ezh2-dependent H3k27_me3.pdf
Tamaño:
1.24 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO COMPLETO EN INGLES
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
FCE - Artículos de Revista