Aislamiento de un bacteriófago para Fusobacterium nucleatum y evaluación de su capacidad lítica en biopelículas
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2012
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es
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Universidad Andrés Bello
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Licencia CC
Resumen
Aislamiento de un bacteriófago para Fusobacterium nuc/eatum y evaluación de su
capacidad lítica en biopelículas.
Actualmente existe una gran variedad de patologías cuyos tratamientos resultan
insatisfactorios debido a la aparición de bacterias resistentes a antibióticos. Por esta
razón se ha retomado la fagoterapia, la cual consiste en utilizar bacteriófagos, que son
pequeñas partículas virales que infectan bacterias. Objetivo: "Aislar desde saliva un
nuevo bacteriófago especifico para F. nuc/eatum y evaluar su capacidad lítica cuando
esta bacteria se encuentra formando biopelículas simples".
Metodologías:
• Aislar el bacteriófago: A partir de muestras de saliva de pacientes con y sin
enfermedad periodontal.
• Evaluar la especificidad del bacteriófago: Se determinó por el crecimiento
homogéneo o la inhibición de éste en la zona de inoculación del bacteriófago.
• Formar biopelícula simple de F. nucleatum in vitro: Se utilizaron placas
multipocillo que se incubaron en anaerobiosis para permitir la formación de
biopelículas que se revelaron con cristal violeta.
• Evaluar la capacidad lítica del bacteriófago sobre biopelícula simple de F.
nuc/eatum: Se realizó el recuento de viabilidad bacteriana y la medición de la
biopelícula, una vez inoculado el bacteriófago.
• Tipo de estudio: Analítico experimental, inserto en el Área de Investigación
delas Ciencias Odontológicas y en la línea de investigación, Etiopatogenia de las
enfermedades bucales.
• Análisis estadísticos a utilizar: Se realizó una estadística descriptiva con un
programa computacional estandarizado.
Resultados: Se aisló un nuevo bacteriófago Fnn<P107 que generó la lisis de
Fusobacterium nuc/eatum cuando este formaba parte de un biopelícula simple.
Discusión: Según estos resultados se promueve continuar investigando el posible uso
de los bacteriófagos como una alternativa terapéutica complementaria a los
tratamientos actualmente disponibles para la enfermedad periodontal.
Conclusiones: Se logró el aislamiento de un nuevo bacteriófago específico para
Fusobacterium nuc/eatum. El bacteriófago Fnn<P107 disminuye significativamente en un
77,68% el recuento UFC/ml de F. nucleatum, cuando se encuentra formando
biopelícula simple.
lsolation of a bacteriophage for Fusobacterium nuc/eatum and evaluation of its lytic capacity within biofilms. Currently there are a variety of diseases whose treatments are unsatisfactory due to the emergence of antibiotic resistant bacteria. Therefore phagotherapy has resumed, which is to use bacteriophages, which are small viral particles that infect bacteria. Objective: "lsolate from spit a new bacteriophage specific for F. nucleatum and evaluate their lytic ability when these bacteria within simple biofilm. " Methodologies: • lsolate the bacteriophage: Since saliva samples from patients with and without periodontal disease. • Evaluate bacteriophage specificity: lt was determined by growth inhibition of homogeneous or this area bacteriophage inoculation. • Form a Simple biofilm in vitro for F. nuc/eatum: We used multiwell plates were incubated in anaerobiosis to allow formation of biofilms that are revealed with crystal violet. • Evaluate the bacteriophage lytic ability within simple biofilm of F. nuc/eatum: count was performed and measuring bacteria! viability of the biofilm, once inoculated bacteriophage. • Type of study: Analytical experimental insert in Area Dental Sciences and Research in the research, Pathogenesis of oral diseases. • Statistical analysis used: descriptive statistics was conducted with a standardized computer program. Results: We isolated a new bacteriophage Fnn<t>107 that gen re lysis of Fusobacterium nuc/eatum when this was part of a simple biofilm. Discussion: Our results supports the further investigation is the possible use of bacteriophages as a complementary therapeutic alternative to currently available treatments for periodontal disease. Conclusions: We achieved the isolation of a new bacteriophage specific for Fusobacterium nuc/eatum. The bacteriophage Fnn<t>107 decreases significantly by 77.68% counting UFC / ml F. nuc/eatum, when within simple biofilm .
lsolation of a bacteriophage for Fusobacterium nuc/eatum and evaluation of its lytic capacity within biofilms. Currently there are a variety of diseases whose treatments are unsatisfactory due to the emergence of antibiotic resistant bacteria. Therefore phagotherapy has resumed, which is to use bacteriophages, which are small viral particles that infect bacteria. Objective: "lsolate from spit a new bacteriophage specific for F. nucleatum and evaluate their lytic ability when these bacteria within simple biofilm. " Methodologies: • lsolate the bacteriophage: Since saliva samples from patients with and without periodontal disease. • Evaluate bacteriophage specificity: lt was determined by growth inhibition of homogeneous or this area bacteriophage inoculation. • Form a Simple biofilm in vitro for F. nuc/eatum: We used multiwell plates were incubated in anaerobiosis to allow formation of biofilms that are revealed with crystal violet. • Evaluate the bacteriophage lytic ability within simple biofilm of F. nuc/eatum: count was performed and measuring bacteria! viability of the biofilm, once inoculated bacteriophage. • Type of study: Analytical experimental insert in Area Dental Sciences and Research in the research, Pathogenesis of oral diseases. • Statistical analysis used: descriptive statistics was conducted with a standardized computer program. Results: We isolated a new bacteriophage Fnn<t>107 that gen re lysis of Fusobacterium nuc/eatum when this was part of a simple biofilm. Discussion: Our results supports the further investigation is the possible use of bacteriophages as a complementary therapeutic alternative to currently available treatments for periodontal disease. Conclusions: We achieved the isolation of a new bacteriophage specific for Fusobacterium nuc/eatum. The bacteriophage Fnn<t>107 decreases significantly by 77.68% counting UFC / ml F. nuc/eatum, when within simple biofilm .
Notas
Tesis (Cirujano Dentista, Especialización en Microbiología Oral)
Palabras clave
Enfermedades de la boca, Tratamiento endodóntico, Microbiología oral, Chile