Neurochemical and behavioral characterization of neuronal glutamate transporter EAAT3 heterozygous mice
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Fecha
2017
Profesor/a Guía
Facultad/escuela
Idioma
en
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Título del volumen
Editor
Sociedad de Biología de Chile
Nombre de Curso
Licencia CC
Licencia CC
Resumen
BACKGROUND: Obsessive-compulsive disorder (OCD) is a severe neuropsychiatric condition affecting 1-3% of the worldwide population. OCD has a strong genetic component, and the SLC1A1 gene that encodes neuronal glutamate transporter EAAT3 is a strong candidate for this disorder. To evaluate the impact of reduced EAAT3 expression in vivo, we studied male EAAT3 heterozygous and wild-type littermate mice using a battery of behavioral paradigms relevant to anxiety (open field test, elevated plus maze) and compulsivity (marble burying), as well as locomotor activity induced by amphetamine. Using high-performance liquid chromatography, we also determined tissue neurotransmitter levels in cortex, striatum and thalamus-brain areas that are relevant to OCD.
RESULTS: Compared to wild-type littermates, EAAT3 heterozygous male mice have unaltered baseline anxiety-like, compulsive-like behavior and locomotor activity. Administration of acute amphetamine (5 mg/kg intraperitoneally) increased locomotion with no differences across genotypes. Tissue levels of glutamate, GABA, dopamine and serotonin did not vary between EAAT3 heterozygous and wild-type mice.
CONCLUSIONS: Our results indicate that reduced EAAT3 expression does not impact neurotransmitter content in the corticostriatal circuit nor alter anxiety or compulsive-like behaviors.
Notas
Indexación: Scopus.
Palabras clave
EAAT3, Neuronal glutamate transporter, Obsessive–compulsive disorder, SLC1A1
Citación
Biological Research. Volume 50, Issue 1, 19 September 2017, Page 29