mRNA-seq reveals skeletal muscle atrophy in response to handling stress in a marine teleost, the red cusk-eel (Genypterus chilensis)

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dc.contributor.authorAedo, Jorge E.
dc.contributor.authorMaldonado, Jonathan
dc.contributor.authorAballai, Víctor
dc.contributor.authorEstrada, Juan M
dc.contributor.authorBastias-Molina, Macarena
dc.contributor.authorMeneses, Claudio
dc.contributor.authorGallardo-Escarate, Cristian
dc.contributor.authorSilva, Herman
dc.contributor.authorMolina, Alfredo
dc.contributor.authorValdés, Juan A.
dc.date.accessioned2023-02-13T13:05:24Z
dc.date.available2023-02-13T13:05:24Z
dc.date.issued2015-12
dc.descriptionIndexación: Scopuses
dc.description.abstractBackground: Fish reared under intensive conditions are repeatedly exposed to stress, which negatively impacts growth. Although most fish follow a conserved pattern of stress response, with increased concentrations of cortisol, each species presents specificities in the cell response and stress tolerance. Therefore, culturing new species requires a detailed knowledge of these specific responses. The red cusk-eel (Genypterus chilensis) is a new economically important marine species for the Chilean aquaculture industry. However, there is no information on the stress- and cortisol-induced mechanisms that decrease skeletal muscle growth in this teleost. Results: Using Illumina RNA-seq technology, skeletal muscle sequence reads for G. chilensis were generated under control and handling stress conditions. Reads were mapped onto a reference transcriptome, resulting in the in silico identification of 785 up-regulated and 167 down-regulated transcripts. Gene ontology enrichment analysis revealed a significant upregulation of catabolic genes associated with skeletal muscle atrophy. These results were validated by RT-qPCR analysis for ten candidates genes involved in ubiquitin-mediated proteolysis, autophagy and skeletal muscle growth. Additionally, using a primary culture of fish skeletal muscle cells, the effect of cortisol was evaluated in relation to red cusk-eel skeletal muscle atrophy. Conclusions: The present data demonstrated that handling stress promotes skeletal muscle atrophy in the marine teleost G. chilensis through the expression of components of the ubiquitin-proteasome and autophagy-lysosome systems. Furthermore, cortisol was a powerful inductor of skeletal muscle atrophy in fish myotubes. This study is an important step towards understanding the atrophy system in non-model teleost species and provides novel insights on the cellular and molecular mechanisms that control skeletal muscle growth in early vertebrates. © 2015 Aedo et al.es
dc.description.urihttps://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-015-2232-7
dc.identifier.doi10.1186/s12864-015-2232-7
dc.identifier.issn1471-2164
dc.identifier.urihttps://repositorio.unab.cl/xmlui/handle/ria/46770
dc.language.isoenes
dc.publisherBioMed Central Ltd.es
dc.rights.licenseAtribución 4.0 Internacional (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es
dc.subjectCortisoles
dc.subjectGenypterus chilensises
dc.subjectHandling stresses
dc.subjectmRNA-seqes
dc.subjectRed cusk-eeles
dc.subjectSkeletal muscle atrophyes
dc.titlemRNA-seq reveals skeletal muscle atrophy in response to handling stress in a marine teleost, the red cusk-eel (Genypterus chilensis)es
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BMC Genomics Volume 16, Issue 1December 01, 2015 Article number 1024
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