Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide

Vista sencilla de ítem
dc.contributor.authorRiveros, A.
dc.contributor.authorEggeling, C.
dc.contributor.authorRiquelme, S.
dc.contributor.authorAdura, C.
dc.contributor.authorLópez-Iglesias, C.
dc.contributor.authorGuzmán, F.
dc.contributor.authorAraya, E.
dc.contributor.authorAlmada, M.
dc.contributor.authorJuárez, J.
dc.contributor.authorValdez, M.
dc.contributor.authorFuentevilla, I.
dc.contributor.authorLópez, O.
dc.date.accessioned2021-09-22T15:02:54Z
dc.date.available2021-09-22T15:02:54Z
dc.date.issued2020
dc.descriptionIndexación: Scopus.es
dc.description.abstractGold nanorods are highly reactive, have a large surface-to-volume ratio, and can be functionalized with biomolecules. Gold nanorods can absorb infrared electromagnetic radiation, which is subsequently dispersed as local heat. Gold nanoparticles can be used as powerful tools for the diagnosis and therapy of different diseases. To improve the biological barrier permeation of nanoparticles with low cytotoxicity, in this study, we conjugated gold nanorods with cell-penetrating peptides (oligoarginines) and with the amphipathic peptide CLPFFD. Methods: We studied the interaction of the functionalized gold nanorods with biological membrane models (liposomes) by dynamic light scattering, transmission electron microscopy and the Langmuir balance. Furthermore, we evaluated the effects on cell viability and permeability with an MTS assay and TEM. Results and Discussion: The interaction study by DLS, the Langmuir balance and cryo-TEM support that GNR-Arg7 CLPFFD enhances the interactions between GNRs and biological membranes. In addition, cells treated with GNR-Arg7 CLPFFD internalized 80% more nanoparticles than cells treated with GNR alone and did not induce cell damage. Conclusion: Our results indicate that incorporation of an amphipathic sequence into oligoarginines for the functionalization of gold nanorods enhances biological membrane nanoparticle interactions and nanoparticle cell permeability with respect to nanorods functionalized with oligoarginine. Overall, functionalized gold nanorods with amphipathic arginine rich peptides might be candidates for improving drug delivery by facilitating biological barrier permeation.es
dc.description.urihttps://www.dovepress.com/improving-cell-penetration-of-gold-nanorods-by-using-an-amphipathic-ar-peer-reviewed-fulltext-article-IJN
dc.identifier.citationInternational Journal of Nanomedicine, Volume 15, Pages 1837 - 1851, 2020es
dc.identifier.doiDOI: 10.2147/IJN.S237820
dc.identifier.issn1176-9114
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/20354
dc.language.isoenes
dc.publisherDove Medical Press Ltd.es
dc.rights.licenseAttribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.subjectAmphipathic arginine rich peptidees
dc.subjectBiological barrier permeationes
dc.subjectCell-penetrating peptideses
dc.subjectGold nanorods; Liposomees
dc.subjectCell-Penetrating Peptideses
dc.subjectPenetratines
dc.subjectOctaargininees
dc.titleImproving cell penetration of gold nanorods by using an amphipathic arginine rich peptidees
dc.typeArtículoes
Archivos
Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Riveros_Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide.pdf
Tamaño:
13.33 MB
Formato:
Adobe Portable Document Format
Descripción:
TEXTO_COMPLETO_EN_INGLES
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar
Colecciones
FCE - Artículos de Revista