Tellurite enters Escherichia coli mainly through the PitA phosphate transporter
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Archivos
Fecha
2012-09
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Wiley
Nombre de Curso
Licencia CC
Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)
Licencia CC
Resumen
everal transporters suspected to be involved in tellurite uptake in Escherichia coli were analyzed. Results showed that the PitA phosphate transporter was related to tellurite uptake. Escherichia coli ΔpitA was approximately four-fold more tolerant to tellurite, and cell viability remained almost unchanged during prolonged exposure to the toxicant as compared with wild type or ΔpitB cells. Notably, reduced thiols (toxicant targets) as well as superoxide dismutase, catalase, and fumarase C activities did not change when exposing the ΔpitA strain to tellurite, suggesting that tellurite-triggered oxidative damage is attenuated in the absence of PitA. After toxicant exposure, remaining extracellular tellurite was higher in E. coli ΔpitA than in control cells. Whereas inductively coupled plasma atomic emission spectrometric studies confirmed that E. coli ΔpitA accumulates ~50% less tellurite than the other strains under study, tellurite strongly inhibited 32Pi uptake suggesting that the PitA transporter is one of the main responsible for tellurite uptake in this bacterium.
Notas
Indexación: Scopus.
Palabras clave
Escherichia coli, Phosphate transport, PitA, Tellurite uptake
Citación
Microbiology, Volume 1, Issue 3, Pages 259 - 267, September 2012
DOI
10.1002/mbo3.26