Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum
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Fecha
2021-12
Profesor/a Guía
Facultad/escuela
Idioma
en
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Título del volumen
Editor
BioMed Central Ltd
Nombre de Curso
Licencia CC
Atribution 4.0 International (CC BY 4.0)
Licencia CC
https://creativecommons.org/licenses/by/4.0/deed.es
Resumen
Background: The native potatoes (Solanum tuberosum subsp. tuberosum L.) grown in Chile (Chiloé) represent a new,
unexplored source of endophytes to fnd potential biological control agents for the prevention of bacterial diseases,
like blackleg and soft rot, in potato crops.
Result: The objective of this study was the selection of endophytic actinobacteria from native potatoes for antago‑
nistic activity against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum, and their
potential to suppress tissue maceration symptoms in potato tubers. This potential was determined through the
quorum quenching activity using a Chromobacterium violaceaum ATCC 12472 Wild type (WT) bioassay and its colo‑
nization behavior of the potato plant root system (S. tuberosum) by means of the Double labeling of oligonucleotide
probes for fuorescence in situ hybridization (DOPE-FISH) targeting technique. The results showed that although Strep tomyces sp. TP199 and Streptomyces sp. A2R31 were able to inhibit the growth of the pathogens, only the Streptomyces
sp. TP199 isolate inhibited Pectobacterium sp. growth and diminished tissue maceration in tubers (p≤0.05). Streptomy ces sp. TP199 had metal-dependent acyl homoserine lactones (AHL) quorum quenching activity in vitro and was able
to colonize the root endosphere 10 days after inoculation.
Conclusions: We concluded that native potatoes from southern Chile possess endophyte actinobacteria that are
potential agents for the disease management of soft rot and blackleg.
Notas
Indexación: Scopus.
Palabras clave
Endophytic Actinobacteria, Streptomyces sp., Quorum Quenching, Confocal Laser Microscopy, Blackleg, Soft Rot, Potato
Citación
BMC Microbiology. Volume 21, Issue 1. December 2021. Article number 335
DOI
10.1186/s12866-021-02393-x