Modified intracellular-associated phenotypes in a recombinant Salmonella typhi expressing S. typhimurium SPI-3 sequences
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Fecha
2010-02-24
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Public Library of Science
Nombre de Curso
Licencia CC
CC BY 4.0 DEED
Atribución 4.0 Internacional
Licencia CC
https://creativecommons.org/licenses/by/4.0/deed.es
Resumen
A bioinformatics comparison of Salmonella Pathogenicity Island 3 sequences from S. Typhi and S. Typhimurium serovars showed that ten genes are highly conserved. However three of them are pseudogenes in S. Typhi. Our aim was to understand what functions are lost in S. Typhi due to pseudogenes by constructing a S. Typhi genetic hybrid carrying the SPI-3 region of S. Typhimurium instead of its own SPI-3. We observed that under stressful conditions the hybrid strain showed a clear impairment in resistance to hydrogen peroxide and decreased survival within U937 culture monocytes. We hypothesized that the marT-fidL operon, encoded in SPI-3, was responsible for the new phenotypes because marT is a pseudogen in S. Typhi and has a demonstrated role as a transcriptional regulator in S. Typhimurium. Therefore we cloned and transferred the S. Typhimurium marT-fidL operon into S. Typhi and confirmed that invasion of monocytes was dramatically decreased. Finally, our findings suggest that the genomic and functional differences between SPI-3 sequences have implications in the host specificity of Typhi and Typhimurium serovars. Copyright: © 2010 Retamal et al.
Notas
INDEXACIÓN: SCOPUS.
Palabras clave
Anaerobiosis, Gene Expression Regulation, Bacterial, Gene Order, Genes, Bacterial, Genomic Islands, Genotype, Humans, Hydrogen Peroxide, Hydrogen-Ion Concentration, Microbial Viability, Mutation, Operon, Phenotype, Reverse Transcriptase Polymerase Chain Reaction, Salmonella typhi, Salmonella typhimurium, Temperature, Temperature, Transformation, Genetic, U937 Cells
Citación
PLoS ONE, Volume 5, Issue 2, 24 February 2010, Article number e9394
DOI
10.1371/journal.pone.0009394