Efecto antimicrobiano de alcoholes volĂĄtiles sobre microorganismos comĂșnmente utilizados en estudios de sensibilidad antimicrobiana
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Fecha
2023
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Facultad/escuela
Idioma
es
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Universidad Andrés Bello
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Licencia CC
Licencia CC
Resumen
Aunque existen compuestos ampliamente utilizados como antisépticos y/o
desinfectantes en diversos campos, Ă©stos en general presentan usos especĂficos
y limitaciones en el ĂĄrea clĂnica o cosmĂ©tica. Por esto la bĂșsqueda de nuevos
agentes con propiedades antimicrobianas siguen siendo necesaria. Desde que
fueron descubiertos, los alcoholes volĂĄtiles han sido empleados en campos
diversos, tales como el ĂĄrea de la medicina debido a su rĂĄpida acciĂłn y amplio
espectro de acciĂłn sobre hongos, virus y bacterias Gram positivo y Gram
negativo. En este estudio se evaluĂł la capacidad antimicrobiana de un set de
alcoholes volĂĄtiles de origen vegetal contra los microorganismos Escherichia coli,
Staphylococcus aureus, Pseudomonas sp., Bacillus cereus y la levadura Candida
albicans. Estos cinco microorganismos son usualmente utilizados para realizar
pruebas de capacidad antimicrobiana de compuestos de uso comercial y/o
clĂnico. De esta forma, la pregunta de la investigaciĂłn es âÂżLos alcoholes
volĂĄtiles analizados presentan un efecto antimicrobiano relevante contra las
bacterias y levaduras empleadas en estudios antimicrobianos?â y el principal
objetivo es âEvaluar la actividad antimicrobiana de los alcoholes volĂĄtiles
analizados contra bacterias y levaduras comĂșnmente utilizadas en estudios de
sensibilidad antimicrobiana, en comparaciĂłn con el antimicrobiano Clorhexidinaâ.
Para realizar lo anterior, se efectuarĂĄn ensayos tipo antibiograma en cultivo sĂłlido
y la determinaciĂłn de la concentraciĂłn mĂnima inhibitoria y la concentraciĂłn
mĂnima bactericida para cada alcohol sobre cada microorganismo, para luego
realizar una comparaciĂłn relativa con los resultados obtenidos con la clorhexidina
como antimicrobiano estĂĄndar. Como conclusiĂłn podemos definir a los alcoholes
PEA, Citronelal, CinamaldehĂdo y Carvacrol con una efectividad antimicrobiana
relevante y por tanto con un potencial uso como agente activo para el control
microbiano.
Although there are compounds widely used as antiseptics and/or disinfectants in various fields, they generally have specific uses and limitations in the clinical or cosmetic area. For this reason, the search for new agents with antimicrobial properties is still necessary. Since they were discovered, volatile alcohols have been used in various fields, such as medicine, due to their rapid action and broad spectrum of action on fungi, viruses, and Gram-positive and Gram-negative bacteria. In this study, the antimicrobial capacity of a set of volatile alcohols of plant origin was evaluated against Escherichia coli, Staphylococcus aureus, Pseudomonas sp., Bacillus cereus and Candida albicans yeast microorganisms. These five microorganisms are usually used to test the antimicrobial capacity of compounds for commercial and/or clinical use. Thus, the research question is "Do the volatile alcohols analyzed present a relevant antimicrobial effect against the bacteria and yeasts used in antimicrobial studies?" and the main objective is "To evaluate the antimicrobial activity of the volatile alcohols analyzed against bacteria and yeasts commonly used in antimicrobial susceptibility studies, in comparison with the antimicrobial Chlorhexidine". To carry out the above, antibiogram type tests will be carried out in solid culture and the determination of the minimum inhibitory concentration and the minimum bactericidal concentration for each alcohol on each microorganism, to then make a relative comparison with the results obtained with chlorhexidine as a standard antimicrobial. As a conclusion, we can define the alcohols PEA, Citronella, Cinnamaldehyde and Carvacrol with a relevant antimicrobial effectiveness and therefore with a potential use as an active agent for microbial control.
Although there are compounds widely used as antiseptics and/or disinfectants in various fields, they generally have specific uses and limitations in the clinical or cosmetic area. For this reason, the search for new agents with antimicrobial properties is still necessary. Since they were discovered, volatile alcohols have been used in various fields, such as medicine, due to their rapid action and broad spectrum of action on fungi, viruses, and Gram-positive and Gram-negative bacteria. In this study, the antimicrobial capacity of a set of volatile alcohols of plant origin was evaluated against Escherichia coli, Staphylococcus aureus, Pseudomonas sp., Bacillus cereus and Candida albicans yeast microorganisms. These five microorganisms are usually used to test the antimicrobial capacity of compounds for commercial and/or clinical use. Thus, the research question is "Do the volatile alcohols analyzed present a relevant antimicrobial effect against the bacteria and yeasts used in antimicrobial studies?" and the main objective is "To evaluate the antimicrobial activity of the volatile alcohols analyzed against bacteria and yeasts commonly used in antimicrobial susceptibility studies, in comparison with the antimicrobial Chlorhexidine". To carry out the above, antibiogram type tests will be carried out in solid culture and the determination of the minimum inhibitory concentration and the minimum bactericidal concentration for each alcohol on each microorganism, to then make a relative comparison with the results obtained with chlorhexidine as a standard antimicrobial. As a conclusion, we can define the alcohols PEA, Citronella, Cinnamaldehyde and Carvacrol with a relevant antimicrobial effectiveness and therefore with a potential use as an active agent for microbial control.
Notas
Tesis (Licenciado en BiologĂa)
Palabras clave
Agentes Antimicrobianos, Alcoholes VolĂĄtiles, Microorganismos