Isolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermispora

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dc.contributor.authorTello, M.
dc.contributor.authorSeelenfreund, D.
dc.contributor.authorLobos, S.
dc.contributor.authorGaskell, J.
dc.contributor.authorCullen, D.
dc.contributor.authorVicuña, R.
dc.date.accessioned2021-05-24T22:04:09Z
dc.date.available2021-05-24T22:04:09Z
dc.date.issued2001-05
dc.descriptionIndexación: Scopus.es
dc.description.abstractGenetic analyses of the lignin-degrading fungus Ceriporiopsis subvermispora is complicated by a dikaryotic nuclear condition and the absence of spore forms. Previous investigations had identified a family of closely related sequences encoding manganese peroxidase (MnP), but the relationship between genes and allelic variants could not be experimentally established. Addressing this issue, homokaryotic derivatives of C. subvermipora strain FP105752 were isolated from regenerated protoplasts. Designated CsA and CsB, their homokaryotic nature was established by polymerase chain reaction amplification and sequence analysis of the allelic variants of three MnP genes. Isoelectrofocusing revealed fewer MnP isoenzymes in filtrates of homokaryon cultures relative to the parental strain. The homokaryotic strains will simplify genetic analyses, particularly the identification of new genes.es
dc.description.urihttps://academic-oup-com.recursosbiblioteca.unab.cl/femsle/article/199/1/91/553870
dc.identifier.citationFEMS Microbiology Letters Volume 199, Issue 1, 15 May 2001, Pages 91-96es
dc.identifier.doiDOI: 10.1016/S0378-1097(01)00156-2
dc.identifier.issn0378-1097
dc.identifier.urihttp://repositorio.unab.cl/xmlui/handle/ria/18930
dc.language.isoenes
dc.publisherFederation of European Microbiological Societieses
dc.subjectBasidiomycetees
dc.subjectCeriporiopsis subvermisporaes
dc.subjectHomokaryones
dc.subjectLigninolytices
dc.subjectManganese peroxidasees
dc.subjectAmino Acid Sequencees
dc.subjectBase Sequencees
dc.subjectBasidiomycotaes
dc.subjectDNA, Fungales
dc.subjectIsoelectric Focusinges
dc.subjectKaryotypinges
dc.subjectLignines
dc.subjectMolecular Sequence Dataes
dc.subjectPeroxidaseses
dc.subjectPolymerase Chain Reactiones
dc.subjectProtoplastses
dc.subjectFungies
dc.titleIsolation and characterization of homokaryotic strains from the ligninolytic basidiomycete Ceriporiopsis subvermisporaes
dc.typeArtículoes
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