Identificación de variedades de arándano (Vaccinium spp.) empleando la técnica de análisis de fusión de alta densidad HRM con tres marcadores moleculares
No hay miniatura disponible
Archivos
Fecha
2023
Autores
Profesor/a Guía
Facultad/escuela
Idioma
es
Título de la revista
ISSN de la revista
Título del volumen
Editor
Universidad Andrés Bello
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Bella, Mágica y Bonita son algunas de las variedades de arándano (Vaccinium spp.) de un alto
interés comercial. Por lo que es indispensable su correcta identificación en etapas tempranas
produciendo dificultades por su fuerte similitud morfológica y su estrecha relación entre ellas. Es
por esto que la implementación de técnicas basadas en secuenciación de DNA son de un gran
interés pero conlleva un alto gasto económico y de tiempo, Es por eso que se plantea implementar
una técnica novedosa llamada fusión de alta resolución de PCR cuantitativa (qPCR-HRM) con el
objetivo de acortar los tiempos de respuesta y gastos asociados a la identificación de variedades
vegetales de Arándano. Los resultados con la técnica de qPCR-HRM se basa en interpretación
de los perfiles de fusión para cada variedad de Arándano empleando marcadores moleculares
polimórficos de tipo SSR o comúnmente llamados microsatélites para la discriminación de
Vaccinium spp. Se trabajó con 11 variedades de Arándano estrechamente relacionadas, esto
implica una alta similitud de los alelos entre las variedades siendo un proceso largo el análisis de
datos para la técnicas actuales de identificación como electroforesis capilar. Dentro de los
resultados de qPCR=HRM el comportamiento de los perfiles o curvas de fusión fue muy
diferenciador donde se logra distinguir claramente unos perfiles de fusión de otros esto
proporcionó información valiosa para la interpretación y análisis de datos integral logrando así
una mayor resolución de los resultados, las curvas de fusión para las 11 variedades de arándano
fueron distintivas pudiendo discriminar entre todos los perfiles de fusión.
Bella, Magic and Bonita are some of the blueberry varieties (Vaccinium spp.) of high commercial interest. Therefore, their correct identification in early stages is essential, causing difficulties due to their strong morphological similarity and their close relationship between them. This is why the implementation of techniques based on DNA sequencing is of great interest but entails high financial and time expenditure. This is why it is proposed to implement a novel technique called high-resolution fusion of quantitative PCR (qPCR-HRM). with the aim of shortening response times and expenses associated with the identification of Blueberry plant varieties. The results with the qPCR-HRM technique are based on the interpretation of the fusion profiles for each Blueberry variety using polymorphic molecular markers of the SSR type or commonly called microsatellites for the discrimination of Vaccinium spp. We worked with 11 closely related Blueberry varieties, this implies a high similarity of the alleles between the varieties, making the data analysis for current identification techniques such as capillary electrophoresis a long process. Within the qPCR=HRM results, the behavior of the melting profiles or curves was very differentiating where it was possible to clearly distinguish some melting profiles from others, this provided valuable information for the interpretation and analysis of comprehensive data, thus achieving greater resolution of the results. Results, the melting curves for the 11 blueberry varieties were distinctive and could discriminate between all melting profiles.
Bella, Magic and Bonita are some of the blueberry varieties (Vaccinium spp.) of high commercial interest. Therefore, their correct identification in early stages is essential, causing difficulties due to their strong morphological similarity and their close relationship between them. This is why the implementation of techniques based on DNA sequencing is of great interest but entails high financial and time expenditure. This is why it is proposed to implement a novel technique called high-resolution fusion of quantitative PCR (qPCR-HRM). with the aim of shortening response times and expenses associated with the identification of Blueberry plant varieties. The results with the qPCR-HRM technique are based on the interpretation of the fusion profiles for each Blueberry variety using polymorphic molecular markers of the SSR type or commonly called microsatellites for the discrimination of Vaccinium spp. We worked with 11 closely related Blueberry varieties, this implies a high similarity of the alleles between the varieties, making the data analysis for current identification techniques such as capillary electrophoresis a long process. Within the qPCR=HRM results, the behavior of the melting profiles or curves was very differentiating where it was possible to clearly distinguish some melting profiles from others, this provided valuable information for the interpretation and analysis of comprehensive data, thus achieving greater resolution of the results. Results, the melting curves for the 11 blueberry varieties were distinctive and could discriminate between all melting profiles.
Notas
Memoria de título (Ingeniero en Biotecnología)
Palabras clave
Arándanos, Identificación, Fusión de Alta Resolución