In vitro genomic and proteomic evidence of a type IV pili-like structure in the fish pathogen Piscirickettsia salmonis
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Fecha
2018-08
Profesor/a Guía
Facultad/escuela
Idioma
en
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Título del volumen
Editor
Oxford University Press
Nombre de Curso
Licencia CC
CC BY 4.0
Licencia CC
Resumen
Piscirickettsia salmonis is an intracellular γ -proteobacteria and the etiological agent of piscirickettsiosis, which causes massive economic losses in the Chilean salmon industry. The type IV pili (T4P) play an important role in adherence to host cell surfaces and bacterial pathogenicity. T4P contains a variable number of components, as predicted in P. salmonis genomes. However, no studies have determined if P. salmonis possesses T4P. The aims of this investigation were to identify T4P components in the P. salmonis type strain LF-89T, evaluate respective transcript expressions, and analyze the main putative T4P proteins using bioinformatics and proteomic approaches. Two main clusters of P. salmonis T4P genes were found. Expression of the pilA gene was upregulated at 4 h post-infection (hpi), while pilQ was upregulated 4 days post-infection. At 16 hpi, pilB and pilD were strongly upregulated. The PilA amino acid sequence analysis showed a conserved N-terminal domain and sequence motifs critical for T4P biosynthesis. MudPIT analysis revealed PilA in the P. salmonis LF-89T proteome, and TEM showed pili-like filamentous structures on the P. salmonis surface. These results strongly suggest the presence of a T4P-like structure in P. salmonis. © FEMS 2018.
Notas
Indexación Scopus
Palabras clave
Fimbriae, Type II Secretion System, Twitching, Piscirickettsia salmonis, Piscirickettsiosis
Citación
FEMS Microbiology Letters Volume 365, Issue 161 August 2018 Article number fny169
DOI
10.1093/femsle/fny169