Evaluación del efecto fenotípico y celular provocado por la infección de flavobacterium psychrophilum en el pez cebra
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Fecha
2017
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Profesor/a Guía
Facultad/escuela
Idioma
es
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Universidad Andrés Bello
Nombre de Curso
Licencia CC
Licencia CC
Resumen
En la última década, la acuicultura ha tenido un importante crecimiento siendo la
competencia directa de la pesca extractiva. Se estima que para el año 2030 la
acuicultura aportará el 62% de la producción destinada al consumo humano. En los
últimos años, se ha intensificado el estudio de enfermedades y patógenos de
importancia comercial con el objetivo de controlar o erradicar estas patologías de los
cultivos.
Dentro de las especies más cultivadas, están la trucha arcoíris (Oncorhynchus
mykiss) y el Salmón coho (Oncorhynchus kisutch). Estas especies se ven afectadas por
diversas enfermedades dentro de las cuales encontramos la enfermedad del agua fría
(EAF). Esta enfermedad, se caracteriza por generar lesiones y úlceras en la piel de los
peces en cultivo, las cuales en su estado severo exponen órganos internos provocando
la muerte del individuo infectado.
El agente responsable de EAF es la bacteria Flavobacterium psychrophilum (F.
psychrophilum). Actualmente, se conoce sólo parte del mecanismo de infección, por lo
que parece importante generar más estudios para definir el proceso y progresión de la
infección, disminuyendo la infección en peces.
Un buen modelo de estudio es el pez cebra, es de tamaño pequeño y presenta
un rápido crecimiento. Es versátil en cuanto a las herramientas celulares y moleculares
que se encuentran disponibles. Ha sido utilizado como modelo de infección en otras
enfermedades importantes para la acuicultura.
En este estudio se utilizo al pez cebra como modelo de infección para F.
psychrophilum para evaluar el efecto a nivel fenotípico y celular. Como resultado,
obtuvimos un fenotipo similar a lo descrito para EAF en otras especies, junto con una
activación del sistema inmune innato frente a la presencia de la bacteria. Por tanto, el
pez cebra es un buen modelo de infección para F. psychrophilum, el cual podría ser de
gran importancia, para establecer formas de control y erradicación de este organismo
infeccioso.
In the last decade aquaculture has had an important growth being the direct competitor of extractive fishing. It is estimated that for the year 2030 aquiculture will provide 62% of the production destined to human consumption. In recent years, the study of diseases and pathogens of commercial importance has intensified with the objective of controlling or eradicate these pathologies from the cultivated fish. Within the most cultivated species there are the Rainbow Trout (Oncorhynchus mykiss) and the Coho Salmon (Oncorhynchus kisutch). These species are affected by diverse diseases among which we find the Bacterial cold water disease (BCWD). This disease characterizes itself for generating injuries and ulcers on the skin of the cultivated fish, which in severe state exposes internal organs resulting in the death of the infected subject. The agent responsible of BCWD is Flavobacterium psychrophilum (F. psychrophilum). Nowadays, only part of the infection mechanism is known, therefore, it seems important to develop more studies to define the process and progression of the disease to reduce the infection among the fish. A good research subject is the Zebrafish, which is small in size and grows rapidly. Moreover, It is versatile regarding the cellular and molecular structure it possesses. It has been utilized as a study subject for other important diseases in aquaculture. In this research the zebrafish was used as a test subject for the infection F. psychrophilum to evaluate the effect at a phenotypical and cellular level. As a result, we obtained a similar phenotype to the one described for BCWD in other species, along with the activation of the innate immune system when confronted with the bacteria. Consequently, the zebrafish is a good test subject for the infection F. psychrophilum, which could be of great relevance to stablish ways to control and eradicate this infectious organism.
In the last decade aquaculture has had an important growth being the direct competitor of extractive fishing. It is estimated that for the year 2030 aquiculture will provide 62% of the production destined to human consumption. In recent years, the study of diseases and pathogens of commercial importance has intensified with the objective of controlling or eradicate these pathologies from the cultivated fish. Within the most cultivated species there are the Rainbow Trout (Oncorhynchus mykiss) and the Coho Salmon (Oncorhynchus kisutch). These species are affected by diverse diseases among which we find the Bacterial cold water disease (BCWD). This disease characterizes itself for generating injuries and ulcers on the skin of the cultivated fish, which in severe state exposes internal organs resulting in the death of the infected subject. The agent responsible of BCWD is Flavobacterium psychrophilum (F. psychrophilum). Nowadays, only part of the infection mechanism is known, therefore, it seems important to develop more studies to define the process and progression of the disease to reduce the infection among the fish. A good research subject is the Zebrafish, which is small in size and grows rapidly. Moreover, It is versatile regarding the cellular and molecular structure it possesses. It has been utilized as a study subject for other important diseases in aquaculture. In this research the zebrafish was used as a test subject for the infection F. psychrophilum to evaluate the effect at a phenotypical and cellular level. As a result, we obtained a similar phenotype to the one described for BCWD in other species, along with the activation of the innate immune system when confronted with the bacteria. Consequently, the zebrafish is a good test subject for the infection F. psychrophilum, which could be of great relevance to stablish ways to control and eradicate this infectious organism.
Notas
Tesis (Magister en Biotecnología)
Palabras clave
Pez Cebra, Bacterias