PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis
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Archivos
Fecha
2016-07
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
Blackwell Publishing Ltd
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Summary
Plant development mediated by the phytohormone auxin depends on tightly controlled
cellular auxin levels at its target tissue that are largely established by intercellular and intracellular
auxin transport mediated by PIN auxin transporters. Among the eight members of the
Arabidopsis PIN family, PIN6 is the least characterized candidate.
In this study we generated functional, fluorescent protein-tagged PIN6 proteins and
performed comprehensive analysis of their subcellular localization and also performed a detailed
functional characterization of PIN6 and its developmental roles.
The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and
endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and
Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and
intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates
levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in
auxin transport and homeostasis is required for auxin distribution during lateral and adventitious
root organogenesis and for progression of these developmental processes.
These results illustrate a unique position of PIN6 within the family of PIN auxin transporters
and further add complexity to the developmentally crucial process of auxin transport.
Notas
Indexación: Sopus.
Palabras clave
Auxin, Endoplasmic Reticulum (ER), Lateral Root, PIN, Plasma Membrane (PM)
Citación
New Phytologist. Volume 211, Issue 1, Pages 65 - 74. 1 July 2016
DOI
10.1111/nph.14019