Effect of single post-ovulatory administration of mifepristone (RU486) on transcript profile during the receptive period in human endometrium
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Fecha
2016-04
Profesor/a Guía
Facultad/escuela
Idioma
en
Título de la revista
ISSN de la revista
Título del volumen
Editor
BioScientifica Ltd.
Nombre de Curso
Licencia CC
Licencia CC
Resumen
Progesterone regulates uterine function during the luteal phase and is essential for the acquisition of endometrial receptivity.
The objective of the present study was to identify endometrial transcripts whose expression is altered during the window of implantation
after the administration of 200 mg of the antiprogestin mifepristone, 48 h after the LH peak (LHC2, LHC0ZLH peak), and to determine
the relationship of these transcripts with those regulated during the acquisition of receptivity. Endometrial samples were obtained in
LHC7 from seven women of proven fertility, each one contributing with one cycle treated with placebo and another with mifepristone.
Additionally, endometrial samples were obtained in LHC2 and LHC7 during a single untreated spontaneous cycle from seven normal
fertile women as a reference. DNA microarrays were used to identify transcripts significantly regulated (defined as R2.0-fold change
with false discovery rate below 1% using t-test) with the administration of mifepristone vs placebo, or during the transition from
pre-receptive to receptive (LHC2 vs LHC7). Approximately 2000 transcripts were significantly regulated in both comparisons
(mifepristone vs placebo and LHC2 vs LHC7), but only 777 of them were coincident and displayed opposite regulation except for 25.
The mRNA level for eight selected genes regulated by mifepristone was confirmed by real-time RT-PCR. We conclude that not all changes
in endometrial transcript levels occurring in the transition from LHC2 to LHC7 seem to be regulated by the progesterone receptor and
w37% of the genes whose transcript levels changed by effect of mifepristone could be associated with the acquisition of receptivity.
Reproduction (2016) 151 331–349
Notas
Indexación: Scopus.
Palabras clave
Embryology, Reproductive Medicine, Endocrinology, Obstetrics and Gynecology, Cell Biology
Citación
Reproduction. Volume 151, Issue 4, Pages 331 - 349. April 2016
DOI
10.1530/REP-15-0458